摘要
目的:观察人型支原体(Mh)对人绒毛蜕膜组织的致炎作用。方法:分别用PPLO培养基(阴性对照组)、终浓度为102、104、106CCU/mL的Mh浓缩悬浮液(Mh低、中及高浓度组)及5g/L脂多糖(阳性对照组)刺激人绒毛蜕膜组织0、6、24、36和48h后,ELISA法检测TNF-α、IL-10及前列腺素2(PGE2)的产生情况。结果:刺激6～48h,5组同一时间点及同一组不同时间点人绒毛蜕膜组织TNF-α、IL-10及PGE2生成量差异均有统计学意义(P均<0.001),Mh高浓度组能明显诱导上述3种介质的产生,但PGE2的产生晚于TNF-α和IL-10,与脂多糖效应基本一致。结论:蜕膜组织抗炎效应是非特异性的,低剂量Mh并不能诱导人绒毛蜕膜组织明显的炎症反应。
Aim：To observe the inflammatory reaction in choriodecidua induced by Mycoplasma hominis（Mh）.Methods：Human choriodecidua was stimulated by 102,104,and 106 CCU/mL Mh for 0,6,24,36 and 48 h,then the levels of TNF-α,IL-10 and PGE2 were detected using ELISA method.PPLO was the negtive control and LPS was the positive control.Results：There were significant differences in the yield of TNF-α,IL-10,and PGE2 among the five groups of human choriofecidua incubed by Mh for 6~48 h（P0.001）. Both high-dose Mh and LPS could stimulate choriodecidua to excrete TNF-α,IL-10 and PGE2,but PGE2 appearanced later than TNF-α and IL-10. Conclusion：The anti-inflammatory of choriodecidua is non-specific,and low-dose Mh could not induce any inflammatory response.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2010年第3期438-440,共3页
Journal of Zhengzhou University(Medical Sciences)
关键词
人型支原体
绒毛蜕膜组织
炎症反应
Mycoplasma hominis
choriodecidua tissue
inflammatory response
