摘要
以6株越橘实生苗为材料,去壁低渗火焰干燥法制片后观察记录染色体数,并进行染色体核型分析.实验结果表明:所有实验材料均属2B核型.其中1号材料体细胞染色体数目为48,核型公式为2n=4x=34 m+12sm(2SAT)+2T;2号材料体细胞染色体数目为48,核型公式为2n=4x=48=32 m(2SAT)+10sm(2SAT)+4st+2T;3号材料体细胞染色体数目为48,核型公式为2n=4x=48=38 m(2SAT)+8sm+2T;4号材料体细胞染色体数目为48,核型公式为2n=4x=48=30 m(4SAT)+12sm+6st;5号材料体细胞染色体数目为24,核型公式为2n=2x=24=20 m+4sm(2SAT);6号材料体细胞染色体数目为24,核型公式为2n=2x=24=16 m(2SAT)+6sm+2st.
Mitotic chromosome samples of 6 Vaccinium seedlings,designated as materials 1,2,3,4,5 and 6,were prepared with the wall degradation hypotonic method and their karyotypes were analyzed.The results showed that the karyotypes of all the 6 experimental materials were of Type "2B",being K(2n)=4x=34m+12sm(2SAT)+2T for material 1,K(2n)=4x=48=32m(2SAT)+10sm(2SAT)+4st+2T for material 2,K(2n)=4x=48=38m(2SAT)+8sm+2T for material 3,K(2n)=4x=48=30m(4SAT)+12sm+6st for material 4,K(2n)=2x=24=20m+4sm(2SAT) for material 5 and K(2n)=2x=24=16m(2SAT)+6sm+2st for material 6.
出处
《西南师范大学学报(自然科学版)》
CAS
CSCD
北大核心
2010年第3期70-75,共6页
Journal of Southwest China Normal University(Natural Science Edition)
关键词
越橘
染色体
核型分析
Vaccinium L.
chromosome
karyotype