摘要
以溴化乙锭(ethidium bromide,EB)为荧光探针,研究了丝裂霉素C(mitomycin C,MMC)与小牛胸腺DNA(CT DNA)的作用机制.对荧光光谱、偏振荧光、Scatchard图、DNA热变性曲线等研究的结果表明,经Na_2S_2O_4还原活化后的MMC能与DNA发生作用,并且存在沟槽和部分嵌入两种结合方式.这一结果进一步证实了前人提出的MMC与DNA之间发生交联反应的结论.
In this paper, the interaction mechanism between mitomycin C and calf thymus DNA has been studied by fluorescence method using EB as an extrinsic fluorescence probe. The results show that mitomycin C can interact with DNA only after being reductively activated by Na2S2O4, and the interaction mode between them should be minor groove binding and partial intercalation. This result further proves that mitomycin C interacts with DNA in the mode of cross - linking.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
1999年第2期171-175,共5页
Acta Chimica Sinica
基金
国家自然科学基金(39660080)
江西省自然科学基金
关键词
丝裂霉素C
DNA
溴化乙锭探针
荧光法
mitomycin C, DNA, ethidium bromide probe, fluorescence method
