摘要
以Ｖ氯仿∶Ｖ甲醇∶Ｖ二氯甲烷∶Ｖ正己烷＝２．６∶１．２∶１∶５为展开剂，建立了薄层色谱扫描法测定痕量药物尼莫地平的新方法。其Ｒｆ值为０．４８。扫描波长为３６５ｎｍ，该法的最低检出限为０．００５μｇ，相对标准偏差为２．９４％，工作曲线的线性范围为０．００５～１μｇ。用该法测定了加有尼莫地平的血清和尿样，尼莫地平的平均回收率为９６．７％～１０３％。
A TLC scanning method is described for the determination of nimodipine. A V(chloroform)∶V(methanol)∶V(dichloromethane)∶V(n hexane)=2.6∶1.2∶1∶5 system was used as the developer. The Rf value of nimodipine is 0.48. The detection limit is 0.005 μg. The RSD is 2.94%. The calibration plot is linear in the range of 0.005～1 μg for nimodipine. The method has been applied to analyze serum and urine spiked with nimodipine, the average recoveries are 96.7%～103%.
出处
《分析测试学报》
CAS
CSCD
1999年第1期67-68,共2页
Journal of Instrumental Analysis
基金
山西省自然科学基金
