摘要
研究了同时测定血清、尿样中的咖啡因和茶碱的薄层色谱定量法。在ＧＦ２５４硅胶板上，用乙酸乙酯－氨水（Ｖ乙酸乙酯∶Ｖ氨水＝９６∶４）的二元展开体系能将这两种生物碱有效地分离。其Ｒｆ值为咖啡因０．４６，茶碱０．１６。分离后的试样用薄层色谱扫描仪进行扫描定量，测定波长２７２ｎｍ，线性范围咖啡因０．００９７～１．９４μｇ，茶碱０．００９０～１．８０μｇ。血清及尿样的加标回收率在９５．８％～１０６．５％之间，相对标准偏差２．９５％～３．５０％。本法较为简便、快速。
A new sensitive, fast TLC densitometric method is described for the simultaneous determination of caffeine and theophylline in body fluid. With a thin layer of silica gel GF254 and a mobile phase of ethylacetate-ammonia water(Vethylacetate ∶Vammonia water =96∶4), caffeine and theophylline were separated from each other. The Rf values are 0.46 and 0.16 for caffeine and theophylline, respectively. The separated spots were scanned by a Shimadu CS-9000 scanner at 272 nm. The linear ranges are 0.009 7～1.94 μg for caffeine and 0.009 0～1.80 μg for theophylline. Their recoveries in serum and urine are 95.8%～106.5%, while relative standard deviations (RSD) are 2.95%～3.50%.
出处
《分析测试学报》
CAS
CSCD
1999年第1期46-48,共3页
Journal of Instrumental Analysis
基金
山西省重点学科专项经费资助
