摘要
在ｐＨ４．３左右的Ｂｒｉｔｏｎ－Ｒｏｂｉｎｓｏｎ（Ｂ－Ｒ）缓冲溶液中，茜素红Ｓ（ＡＲＳ）与人血清白蛋白（ＨＳＡ）结合，生成红色的复合物，吸光度值与ＨＳＡ含量呈线性关系。复合物的吸收峰值λｍａｘ为５３０ｎｍ，比ＡＲＳ试剂本身红移１１０ｎｍ。其表观摩尔吸光系数ε＝１．０×１０４Ｌ·ｍｏｌ－１·ｃｍ－１。ＨＳＡ标准曲线在其质量浓度１０～９００ｍｇ／Ｌ间呈线性关系，最低检出限为３ｍｇ／Ｌ。研究了该配合物用于蛋白质分光光度法测定的基本条件。应用该法测定了人血清样品，回收率在９６．９％～１０２．１％范围内。实验表明该反应选择性、重现性和对照性均好，操作简便。
Human serum albumin reacts with alizarin red S to form a red complex in a Britton-Robinson buffer at pH 4.3. The maximum absorption wavelength of the complex λmax is 530 nm with an apparent molar absorptivity ε of 1.0×104 L·mol-1·cm-1. The peak of the complex has a “red shift” of 110 nm as compared with that of the reagent. The linear range of the standard curve is 10～900 mg/L for albumin, while the detection limit is 3 mg/L. The method has been applied to human serum albumin analysis with recoveries of 96.9%～102.1%. Experimental results indicate that the method has good selectivity, reproducibility, simplicity and a wide concentration range.
出处
《分析测试学报》
CAS
CSCD
1999年第1期9-12,共4页
Journal of Instrumental Analysis
基金
国家自然科学基金
