摘要
目的探讨单心室胎儿心肌组织中PTEN、FOXP1基因的表达。方法取3例妊娠26周龄单心室胎儿(单心室组)和正常胎儿(对照组)各3例心室肌组织标本,采用实时荧光定量-PCR技术检测心室肌组织PTEN、FOXP1基因mRNA的表达。结果单心室组PTEN基因的表达量显著高于对照组[(1477.95±93.3881)vs.(320.6969±27.6075)](P<0.01);单心室组FOXP1基因的表达量也显著高于对照组[(1271.4907±167.3863)vs.(376.5057±236.4714)](P<0.05)。结论 PTEN、FOXP1基因在单心室胎儿心肌组织中表达上调可能与单心室的发生有关。
Objective To explore the expressions of PTEN and FOXP1 gene mRNA in the ventricular myocardium of fetus with single ventricle. Methods The total RNAs of ventricular myocardium were extracted from 3 fetuses with single ventricle（group S） and 3 fetuses with normal ventricular myocardium（group N）.All fetuses were 26 weeks gestation age.The levels of PTEN and FOXP1 mRNA expression were detected by real-time quantitative-PCR.Results The expression of PTEN mRNA was higher in group S than that in group N ／[（1477.95±93.3881） vs.（320.6969 ±27.6075）／]（P0.01）.So did the expression of FOXP1 mRNA／[（1271.4907±167.3863） vs.（376.5057±236.4714）／]（P0.05）.Conclusion The up-regulated expressions of PTEN and FOXP1 genes in ventricular myocardium may be related to the development of single ventricle.
出处
《江苏医药》
CAS
CSCD
北大核心
2010年第10期1143-1145,共3页
Jiangsu Medical Journal
基金
国家自然科学基金(30973213)
