摘要
目的研究缺氧对体外培养血管内皮细胞AQP1表达的影响。方法采用脐静脉内皮细胞株,复苏传代后计数稀释,接种于6孔培养板中,细胞贴壁后分组:常氧对照组(5%CO2+95%空气),缺氧(1%O2+5%CO2+94%N2)3 h,6 h,12 h,24 h组,应用免疫细胞化学半定量检测AQP1蛋白水平的表达以及实时荧光定量-PCR法检测AQP1 mRNA的表达。结果AQP1蛋白和AQP1 mRNA的表达在缺氧后呈先升高后降低的趋势。结论缺氧在蛋白和转录水平对脐静脉内皮细胞AQP1的表达有调节作用。
Objective To investigate the expression of AQP1 in endothelial cells under hypoxia conditions in vitro. Methods The cultured human umbilical vein endothelial cells were randomLy assigned to hypoxia groups （exposed to 1% of O2 for 3 h, 6 h, 12 h and 24 h respectively）, and control group（ordinary culture）. The expressions of AQP1 mRNA and protein were detected hy real time PCR and immunohistochemistry respectively. Results The expressions of AQP1 protein and mRNA increased after exposure to hypoxia initially and then decreased afterwards. Conclusion Hypoxia regulates the expression of AQP1 in vascular endothelial cells.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2008年第6期916-920,共5页
Journal of Sichuan University(Medical Sciences)
基金
四川省科技厅攻关重点项目(2006Z09-032)资助
关键词
血管内皮细胞
水通道蛋白
缺氧
实时荧光定量PCR
Human umbilical vein endothelial cell Aquaporin-1 Hypoxia Real time PCR（real time polymerase chain reaction）
