摘要
采用γ射线诱变与高浓度羟脯氨酸（ＨＹＰ）离体选择的方法，获得抗羟脯氨酸（ＨＹＰ）锦橙悬浮细胞系，并再生植株。抗性细胞系比供体细胞系（对照）含有较高的脯氨酸（Ｐｒｏ），有较高的ＰＯＤ与ＣＡＴ活性及较强的抗ＨＹＰ能力，经冷驯化后抗性细胞系的抗寒性比供体细胞提高１．４℃，同时进一步加大了抗性细胞系在抗氧化酶ＳＯＤ、ＣＡＴ活性和脯氨酸（Ｐｒｏ）、可溶性糖含量及水势上与供体的差异。这表明，抗氧化酶活性的提高及渗透调节能力的加强与抗性系抗寒性的增强有关。
Somaclonal tolerant cell line and its regenerants were obtained from suspension culture cell of ovules in sweet orange Jincheng ( Citrus sinensis Osb ) via gamma ray mutagenesis and high pressure selection of hydroxyproline (HYP), which were 1 4 ℃ more cold tolerant than the wild types of suspension cells after cold acclimation Besides proline accumulation , soluble sugars content and antioxygenases SOD and CAT activities were stimulated much higher and water potential became much lower than the wild type after cold acclimation at the suspension cell levels, which indicated that not only proline metabolism, but antioxygenases and osmotic regulation were also involved in its enhancement process on cold tolerance
关键词
柑桔
抗羟脯氨酸
细胞变异系
抗寒性
渗透调节
Citrus
hydroxyproline resistant somaclonal cell line
cold tolerance
osmotic regulation
antioxygenase
