摘要
目的：以３０％Ⅲ度烧伤大鼠胃肠喂养模型，分离、培养肝巨噬细胞（Ｋｕｐｆｅｒｃｅｌ，ＫＣ），以了解早期肠道营养（ＥＦ）对肝巨噬细胞的调理作用。方法：１０５只大鼠随机分为早期喂养组、延迟喂养组及正常对照组。于伤前及伤后１、３、５天观察肝巨噬细胞分泌ＴＮＦ、ＰＧＥ２的变化及肝巨噬细胞吞噬胶体颗粒数量的变化。结果：延迟喂养组较早期喂养组肝巨噬细胞分泌ＴＮＦ、ＰＧＥ２及吞噬胶体颗粒量均明显增加。结论：早期肠道营养可下向调理肝巨噬细胞的过度激活，减少其释放活性介质，对严重烧伤后因炎症反应失控所致的多器官功能障碍综合征的防治可能有重要的理论及实践意义。
Objectives:To investigate whether there is the modulation effect of early enteral feeding on KC of burned rats. Methods:Rats sustained with 30% Ⅲ° flame burns of TBSA were divided randomly into early feeding(EF) group given gastroenteral feeding beginning from 2 hours postburn( n =45),and delayed feeding(DF) group given the same nutritional solutions but starting on PBD3( n =45).Another 15 rats were served as normal controls(NS) without burning. Results:The results showed that TNF and PGE 2 released by KCs increased more markedly in the DF group than in the EF group.The counts and area of KCs phagocytizing colloidal carbon particles was obvious increase in DF group than that of the EF group. Conclusions:We consider that early enteral feeding can modulate the hyperactivity of KCs and reduce its excessive secretion of active mediators.
出处
《肠外与肠内营养》
CAS
1999年第1期26-28,共3页
Parenteral & Enteral Nutrition
关键词
烧伤
肠道营养
肝巨噬细胞
炎性介质
Burns Early enteral feeding Kupffer cell Inflamatory mediators
