摘要
选择硫苷性状差异大,而其他品质性状和农艺性状相近的油菜品系967H和967L为亲本,用RAPD引物对亲本、F1、F2植株进行分子标记,寻找与硫苷性状相连锁的标记.结果表明,从482个RAPD引物筛选出6个在2个亲本之间表现多态性的引物,从这6个引物中筛选出1个在高硫苷基因池和低硫苷基因池之间有稳定差异的引物S268,用S268对F2群体单株进行检验,群体单株只显示3种带型标记,与低硫苷性状连锁的标记S268对低硫苷性状的贡献率为29.51%.
In order to find intelocking RAPD marks for the glucosinolate trait in Brassica napus,967H and 967L were used as materials,which kept similar characteristics in quality and other agronomic traits except for the glucosinolate trait,to study RAPD marks of the parents and their F1 and F2 plants.The results showed that 6 RAPD primers from 482 RAPD primers were detected polymorphism between parents,and one RAPD primer（S268） from the 6 RAPD primers was detected polymorphism between two parents and two DNA bulks.The primer S268 could only display three kinds of different marks.Variant analysis with a single mark indicated that the low glucosinolate traits of 29.51% were contributed by the S268 mark.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第3期254-257,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家"948"计划项目(2006-G04)
