摘要
硒蛋白的组分分析的难题是硒氨基酸在水解中不稳定。Blau指出硒蛋氨酸(SeMet)在6mol/L HCl、110℃、7h水解中几乎全部被破坏,Shepherd也得到同样的结论。硒胱氨酸和硒半胱氨酸也有相似的行为。其后,前人曾提出各种方法对此加以改进,例如加入各种还原剂以及通氮除氧,目的是防止硒氨基酸被氧化成亚砜或砜。最近刘曼西等提出在4mol/LHCl、50℃,50h的水解方法,但未通氮除氧。
A modified hydrolysis method ( 4mol/L HC1,50℃,50h,in N2) for determining selenoprotein has been established.The recovery of this method is 84% with respect to the bound selenomethionine.The reasons for the comparatively high recovery of this method were found by experiments.This method would be of significance to the determination of selenoprotein.
出处
《色谱》
CAS
CSCD
北大核心
1990年第4期250-251,共2页
Chinese Journal of Chromatography
