摘要
以东北刺人参(Oplopanax elatus Nakai.)无根组培苗为试材,应用"促成休眠"法促使东北刺人参无根组培苗在试管内落叶进入休眠状态,并应用均匀设计法筛选其最适宜的种质试管保存的ABA和KT质量浓度。结果表明,最佳的种质试管保存培养基为:N-68+ABA2.37mg/L+KT0.60mg/L,休眠率达87.8%。常温自然光条件下,利用该法在试管内保存东北刺人参种质资源可达37个月。休眠芽解除休眠后仍可很快萌发并迅速生长。
An experiment was conducted to induce the in vitro shoots of Oplopanax elatus Nakai. into dormancy by the method of promoting dormancy. The suitable concentrations of ABA and KT supplemented into the medium for in vitro germplasm conservation were determined by uniform design. Results showed that N-68 basic medium with 2.37mg/L ABA and 0.60mg/L KT was fit for in vitro germplasm preservation,with a dormancy rate of 87.8%. The conservation period for germplasm of O. elatus could last for 37 months at normal temperature and natural light condition by the method of promoting dormancy. The dormant shoots could germinate and grow rapidly after the dormancy was relieved.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2010年第3期126-127,共2页
Journal of Northeast Forestry University
基金
国家科技攻关计划引导项目(2005BA741C)资助
关键词
东北刺人参
脱落酸
均匀设计
种质试管保存
Oplopanax elatus
Abscisic acid
Uniform design
In vitro germplasm conservation
