摘要
目的:研究两种方法复制家兔急性肾功能衰竭(ARF)模型的成功率、稳定性及应用。方法:皮下注射1%HgCl2(1.5mL/kg.bw,H组)、肌肉注射50%甘油(10mL/kg.bw,G组)复制家兔ARF模型,测定24h和48h血清尿素氮(BUN)、肌酐(Cre)水平和尿常规检查,观察比较各组模型复制的成功率、存活率及稳定性。结果:两组均能成功复制ARF模型;G组BUN、Cre较H组低;尿常规检查均可见蛋白尿和肾衰管型,但G组程度轻;G组的存活率及稳定性显著高于H组。结论:两种模型复制方法各有优缺点,应根据不同研究目的选择相应的模型。
Objective：To study the copying method comparison of successful rate,stability and applications on two model of acute renal failure（ARF）in rabbits.Methods：The rabbit models of acute renal failure were copied by hypodermic injection HgCl2（1.5mL/kg·bw, H group）and intramuscular injection 50% glycerinum（10mL/kg·bw,G group）,the BUN and Cre contents in serum and the Urine routine examination were determined at 24 hours and 48 hours. Observed in different groups.Results：It showed that the H group and G and compared successful rate,survival rate and stability of models group were all successful,the serum BUN,Cre of G group were significantly lower than those of the H group.The Urine routine examination inspects the obvious albuminuria and the kidney fades casts,but G group of degrees are light.But the survival rate and stability of G group were significantly increased compared with H group.Conclusion：There are advantages and disadvantages in two ARF model,we should choose corresponding model according to different aim.
出处
《科技创新导报》
2010年第14期6-7,共2页
Science and Technology Innovation Herald
