摘要
目的 分析血水草血根碱致钉螺肝脏蛋白质表达的变化.方法 从血水草粉末中分离血根碱,配成5 mg/L水溶液,每500 ml药液投放50只钉螺,25℃浸泡36 h,解剖活螺并收集肝脏,双向电泳分离血根碱处理组和清水对照组钉螺肝脏蛋白质,ImageMaster 2D 5.0软件分析图谱,筛选差异蛋白质,并用质谱鉴定.结果 血根碱组和清水对照组分别检出（433±14）、（385±12）个蛋白质点;质谱鉴定获得11个差异表达蛋白,分别是原肌球蛋白、假定蛋白XP_533132、肌动蛋白87E、角蛋白6A、β-微管蛋白、线粒体内膜蛋白4样蛋白、角蛋白2、咽侧体抑制激素A前体、ENSANGP00000020184、肌动蛋白-3、ENSANGP00000013943.其中假定蛋白XP_533132、ENSANGP00000013943在血根碱处理组表达下调,其余9个蛋白质在血根碱处理组表达上调.结论 血根碱引起钉螺肝脏蛋白质表达发生改变.
Objective To identify the differentially expressed proteins in the liver of Oncomelania snails induced by Eomeeon chinanthe sanguinarine.Methods Sanguinarine was extracted and purified from the dry powder of Eomecon chinanthe.Oncomelania snails were immersed in 5 mg/L sanguinarine （50 Oneomelania snails per 500 ml）or pure water for 36 h（25℃）and the livers were isolated from live snails.Total liver proteins were extracted and separated by two-dimensional gel electrophoresis.Electrophoretogram was analyzed by Image Master 2D 5.0 software.The difierentially expressed proteins between sanguinarine group and pure water group were selected and analyzed by matrixassisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF-MS）and tandem mass spectrometry sequencing of tryptic peptides.Results In terms of protein spots,433±14 and 385±12 were observed in sanguinarine group and in water group respectively.The eleven identified differentially expressed proteins included tropomyosin,hypothetical protein XP_533132,actin 87E,keratin 6A,beta-tubulin,mitochondrial inner membrane protein isoform 4,keratin 2,allatostatin precursor,ENSANGP00000020184,actin-3 and ENSANGP00000013943.Among them.hypothetical protein XP.533132 and ENSANGP00000013943 were down-regulated and the other nine proteins were up-regulated in sanguinarine group.Conclusion Sanguinarine could alter the expression of proteins in livers of Oncomelania snails.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2010年第6期490-493,共4页
Chinese Journal of Preventive Medicine
基金
基金项目：国家自然科学基金（30771872）
湖南省教育厅项目（08c570）
长沙市科技局重点项目（K0901021-31）
晓庄学院青年优秀人才培养计划（2008-8）
