摘要
采用反相HPLC法测定了宽体金线蛭、光润金线蛭、尖细金线蛭、日本医蛭、菲牛蛭及不同产地的宽体金线蛭中尿嘧啶、黄嘌呤、次黄嘌呤的含量。色谱柱为Shim-pack CLC-ODS C_(18)柱,流动相:0.05mol/l的磷酸氢二铵溶液(pH=8.4),流速:0.8ml/min,检测波长:254nm,回收率:>98.0％。本法准确、快速、重现性好。
Three constituents(uracil,xanthine,hypoxanthine)were isolated from five species of leech and determined by reversed phase HPLC.The column employed was Shim-pack CLC-ODS C18(150mm× 6 mm).The mobile phase was0.05 mol/l ammonium phophate dibasic solution(pH = 8.4) .The flow rate was 0.8 ml/min and detection was effected at 254 nm.This method is accurate,rapid and reproducible. Analytical data for five species and Whitmama pigra samples from different places were given.
出处
《中药材》
CAS
CSCD
1999年第1期8-9,共2页
Journal of Chinese Medicinal Materials
