摘要
单细胞的电化学研究不仅对于电化学而且对于细胞生物学都是一个挑战.本文亚甲蓝化学修饰微铂盘电极吸附捕获单个酵母菌细胞,以循环伏安和交流阻抗方法研究了被吸附细胞的电化学行为。在亚甲蓝的催化下,吸附的酵母菌细胞给出了一个不可逆氧化还原峰,受表面控制.交流阻抗图中获得电极与溶液,溶液-细胞膜,细胞膜上的氧化还原反应,细胞膜与细胞内液之间的阻抗,电容等相关参数,为单细胞的电化学行为的进一步研究奠定基础。
Single cell electrochemistry is a challenge to electrochemistry and cell biology.In the present paper,A single yeast cell was captured on the methylene blue modified Pt disk electrode surface.The electrochemical behavior of the captured single cell was studied by cyclic voltammetry and electrochemical impedance spectroscopy.The captured single yeast cell shows an surface controlled irreversible reduction peak with the catalysis of methylene blue.Some relative parameters such as the resistances of electrode-solution,solution-cell membrane,electrochemical reaction,cell membrane-inner solution of cell,and electrical capacities were obtained from the electrochemical impedance of the single cell,which offers a basis for the further studies of cell electrochemistry.
出处
《沈阳师范大学学报（自然科学版）》
CAS
2010年第2期251-254,共4页
Journal of Shenyang Normal University:Natural Science Edition
基金
国家自然科学基金资助项目(20807563)
国家重点实验室项目(2006-06)
关键词
铂盘微电极
亚甲蓝化学修饰电极
酵母菌单细胞
循环伏安
电化学交流阻抗
platinum disk microelectrode
methylene blue chemical modified electrode
single yest cell
cyclic voltammetry
electrochemical impedance spectroscopy
