摘要
目的:观察骨保护素对内皮细胞数量变化及内皮型一氧化氮合酶(eNOS)的影响,探讨其对内皮细胞的作用。方法:常规培养永生化的人内皮细胞,用不同浓度(0,0·5,2·0,4·0,6·0,8·0μg/L)的骨保护素作用细胞48h后,用细胞计数试剂盒(cellcount kit,CCK-8)来检测细胞的数量;免疫细胞化学检测eNOS蛋白的表达水平,实时定量PCR检测eNOS基因的定量表达。结果:与对照组(0·936±0·146)相比,在8·0μg/L组的内皮细胞增殖量明显增加(1·291±0·200;P<0·05);eNOS蛋白表达在2·0μg/L、4·0μg/L组(P<0·05)和6·0μg/L、8·0μg/L组(P<0·01)均显著增加;而4·0μg/L、6·0μg/L、8·0μg/L组的eNOS mRNA较对照组均呈显著增高(P<0·01)。结论:骨保护素在人体的浓度波动范围内具有促进内皮细胞增殖效应,在抗动脉粥样硬化过程中,可能具有促内皮细胞修复作用,且与浓度梯度有明显的正相关性。
Objective：Observe the effects of osteoprotegerin （OPG） on the changes of endothelial cell numbers and nitric oxide synthase （eNOS）, in order to investigate the influence of OPG on endothelial cells.Methods：The conventional cultures of immortalized human endothelial cells, with different concentration of OPG （0, 0.5, 2.0, 4.0, 6.0, 8.0 μg/L） cultivating for 48 h, were detected the number of cells with the cell count kit （CCK-8）, eNOS protein expression with immunocytochemical method, eNOS mRNA levels with real-time quantitative PCR.Results：compared to the control group （0.936±0.146）, the cell number of 8.0 μg/L group （1.291±0.200） was evidently elevated （P〈0.05）; compared to the control group, eNOS protein expression in 2.0 μg/L, 4.0 μg/L group （P〈0.05） increased significantly, and increased more remarkably in 6.0 μg/L, 8.0 μg/L groups （P〈0.01）; in 4.0 μg/L, 6.0 μg/L, 8.0 μg/L groups eNOS mRNA were significantly increased （P〈0.01） compared to the control group as well. Conclusion：the concentrations of OPG in human ranges promoted endothelial cell proliferation. As the anti-atherosclerotic factor, OPG may promote endothelial cells＇ repairation, and had evidently positive correlation with the concentration gradient.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2010年第4期307-310,共4页
Journal of Clinical Cardiology
基金
国家自然科学基金资助项目(No:30670885)
