摘要
构建肌肉高表达质粒VR/TPO ,将其在体外定量表达 ,并初步了解其体内表达活性。方法 :以重组TPOcDNA为目的基因 ,构建人VR/TPO高效表达质粒 ,用脂质体法将其转染CHO细胞 ,RT PCR法检测mRNA表达 ,ELISA法检测TPO的表达量 ,并注入体内了解其对巨核系造血的影响。结果 :VR/TPO可在CHO中高效表达 ,其表达量超过 pcDNA3TPO ,并初步发现有促进血小板增加作用。 结论 :VR/TPO为一高效表达TPO质粒 ,在体内外都有一定表达活性。
Objective: To obtain the construct of cDNA TPO, in order to transfer and express it in CHO cell and Balb/c mouse. Methods: The eukaryotic muscular expression vector VR1012/TPO was constructed with human recombinant TPO cDNA as the therapy gene to transfect the CHO cell and directly inject it to BALB/C mouse. Results: CHO cells could be transferred with VR/TPO and highly express TPO protein. VR/TPO was found to promote megakaryocytics in BALB/C mouse. Conclusion: VR/TPO is a good vector to express TPO protein and also can be given directly by muscular injection.
出处
《北京医科大学学报》
CSCD
2000年第3期269-271,共3页
Journal of Peking University(Health Sciences)
