摘要
目的:探讨脂联素(APN)在高糖孵育血管内皮细胞中的表达调节及意义。方法:用不同浓度葡萄糖(5.5、11、22及33mM)孵育人脐静脉内皮细胞(HUVECs),2天后收集细胞和上清液。用实时荧光定量RT-PCR方法检测细胞中APN、肿瘤坏死因子α(TNFα)和过氧化物酶体增生物激活受体γ(PPARγ)的mRNA表达,用ELISA方法检测细胞上清液中APN和TNFα分泌情况。结果:随培养液中葡萄糖浓度的升高,HUVECs中APN的mRNA表达及上清中APN蛋白含量降低(p<0.05),TNFα的mRNA表达及上清中TNFα蛋白含量增加(p<0.05),HUVECs中PPARγ的mRNA表达降低(p<0.05),且组间比较差异均有统计学意义(p<0.05)。结论:高糖诱导血管内皮细胞保护性细胞因子APN表达分泌下降,可能是高糖诱导的血管内皮细胞功能损害的重要机制之一。
Objective：To explore the expression and regulation of APN in Human umbilical vein endothelial cells（HUVECs） treated with high glucose.Methods：HUVECs were treated with D-Glucose in different concentrations of 5.5 mM,11 mM,22 mM,and 33 mM,respectively for 2 days,and then cells and supernate accordingly were collected.The expressions levels of APN,TNFα and PPARγ mRNA were detected by the real-time fluorescence quota RT-PCR method.The level of APN and TNFα in cells medium were determined by enzyme-linked immunosorbent assay（ELISA）.Results：With the increasing of glucoses concentrations in medium,the expressions of APN mRNA and the secretion of protein APN from HUVECs was down-regulated（p 0.05） and the expressions of APN mRNA and the secretion of protein TNFα from HUVECs were up-regulated（p 0.05）.At the same time,the expressions of PPARγ mRNA in HUVECs was down-regulated（p 0.05）.Conclusions：APN mRNA expression and protein APN secretion in vascular endothelial cells treated with high glucose were down-regulated,which may be one of important mechanisms of high glucose-induced vascular endothelial cell function damage.
出处
《现代生物医学进展》
CAS
2010年第9期1661-1664,共4页
Progress in Modern Biomedicine
基金
深圳市重点资助项目(200643)资助
关键词
脂联素
人脐静脉内皮细胞
高葡萄糖
Adiponectin
Human umbilical vein endothelial cells
High glucose
