摘要
目的观察氯化镉(CdCl2)溶液腹腔注射对大鼠的急性毒性。方法通过预实验找出CdCl2染毒SD大鼠100%和0%死亡的剂量范围,求出正式试验的剂量比,以此等比级差设6个剂量实验组和1个对照组,每组8只大鼠,观察大鼠CdCl2急性中毒情况,采用改良寇氏法测定CdCl2溶液腹腔注射对大鼠的LD50。结果初步显示实验大鼠绝对致死量为26.00mg/kg,最大耐受剂量为11.54mg/kg,镉中毒大鼠一般在8h内死亡,少数在72h内死亡;CdCl2溶液腹腔注射对大鼠的LD50为18.37mg/kg,95%的可信区间是16.56～20.38mg/kg。结论参照我国职业性接触毒物危害程度分级标准,CdCl2为高度危害毒物,为镉化物的大鼠急性毒性补充了新的科学数据。
Aim To observe the acute toxicity of rats exposed i.p. to cadmium chloride （CdCl2）,and determine the median lethal dose （LD50）. Methods The dose range leading to the death rate 100% and 0% of Sprague-Dawley rats after exposed i.p. to CdCl2,and the rats were randomly divided into 6 experimental groups and 1 control group each consisted of 8 rats. Results The absolute lethal dose was 26.00 mg/kg and maximal tolerance dose was 11.54 mg/kg. The doses in each experimental groups was 26.00,22.10, 18.79, 15.97,13.57 and 11.54 mg/kg in tune. The rats usually died of Cd intoxication within 8 hours and a few of rats died in 72 hours. The LD50 of rats exposed i.p. to CdC12 was 18.37 mg/kg,with a 95% confidence interval of 16.56-20.38 mg/kg. Conclusions The CdCl2 is a kind of high toxic chemicals according to the harmful classification of occupational exposed poison in China,This experiment has offered scientific data for acute toxicity test of cadmium compounds.
出处
《中国热带医学》
CAS
2010年第7期796-797,共2页
China Tropical Medicine
基金
国家自然科学基金资助项目(30771781)
广东省自然科学基金资助项目(06022672)
