摘要
通过回流冷凝法和低温固相法制备了5种稀土、钨酸、1,3-二苯基-4-吡唑甲醛缩异烟肼三元配合物,并通过元素分析,摩尔电导,IR,UV,TG-DTA,TC-ICP,XPS等对其结构和化学组成进行表征,确定其化学组成Na[RE(HL)(WO4)2]·6H2O(HL:1,3-二苯基-4-吡唑甲醛缩异烟肼).采用MTT比色法研究了配合物对K562肿瘤细胞的细胞毒性,结果表明:三元配合物的IC50在25μg/mL左右,并进行了形态学观察.流式细胞术结果表明:配合物对癌细胞最佳凋亡浓度为12.5μg/mL,细胞被配合物阻滞在S期,抑制了细胞的有丝分裂过程.
Using a reflux condensation method and a low-temperature solid phase method,five kinds of new ternary complexes were synthesized with rare earth chloride,tungstic acid and 1,3-diphenyl-4-pyrazole-formyl isoniazidylhydrazone.Their compositions were characterized by elemental analysis,molar conductance,FT-IR,UV-vis,TG-DTA,TC-ICP,and XPS,and confirmed to be：Na[RE（HL）（WO4）2]·6H2O （HL：1,3-diphenyl-4-pyrazole-formyl isoniazidylhydrazone）.MTT was used to study the complexes＇ cytotoxicity against to K562 tumor cells and morphological observation.The results show that the complexes＇ IC50 were 25 μg/mL,flow cytometry results showed that the best concentration apoptosising on cancer cells were 12.5 μg/mL.Cancer cells were arrested in S phase,inhibitting cells＇ mitosis process.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2010年第8期775-780,共6页
Acta Chimica Sinica
基金
国家自然科学基金(No.20671063)
上海市稀土功能材料重点实验室(No.07dz22303)
上海市重点学科建设项目(No.S30406)
上海师范大学重点学科(No.DZL711)
上海师范大学一般科研项目(No.SK200829)资助项目
