摘要
目的对嗜酸乳杆菌的毒性进行研究。方法采用大、小鼠急性毒性试验、Ames试验、小鼠骨髓细胞微核试验、小鼠精子畸形试验和大鼠30 d喂养等对嗜酸乳杆菌进行安全性试验研究。结果急性经口毒性试验表明,大、小鼠灌胃给予嗜酸乳杆菌,最大耐受剂量雌雄两性别均大于20.0 g/kg体重,Ames试验、微核试验和精子畸形试验结果均为阴性。大鼠30 d喂养试验结果表明各项指标均未见明显毒性反应。结论在本次实验条件下,嗜酸乳杆菌未见遗传毒性。由此可初步判定,使用嗜酸乳杆菌是安全可靠的。
Objective To study the toxicity of Lactobacillus acidophilus LA-G80.Method Acute toxicity test of mice,Ames test,micronucleus test of bone marrow PCE cell in mice,sperm abnormality test of mice and feeding for thirty days were used.Result The oral acute toxicity study indicated that the maximum achievable concentration of each sex were all above 20.0 g/kg body weight;The results of genotoxicity tests were all negative,including the results of Ames test,micronucleus test and sperm shape abnormality test.And the feeding test showed that there was no rat died.Conclusion There is no toxicity in the Lactobacillus acidophilus LA-G80 according to the result of this study, which suggests that use of Lactobacillus acidophilus LA-G80 in food is safe.
出处
《中国微生态学杂志》
CAS
CSCD
2010年第5期446-449,共4页
Chinese Journal of Microecology
