摘要
目的:用乙肝病毒(HBV)血清标志物体外抑制实验的方法,观察嗜酸乳杆菌对E抗原(HBeAg)的抑制效果。方法:以HBeAg阳性血清作为实验对象,将其分为实验组和对照组。各实验组分别加入5%、10%和20%浓度的嗜酸乳杆菌菌素混悬液及经过转种的活菌液。各对照组加入相应的空白稀释液。经37℃温育后,用酶联免疫吸附方法(ELISA)检测各组不同稀释度的光密度(OD)值。将各实验组和对照组OD值绘制曲线,观察嗜酸乳杆菌对HBeAg的抑制效果及其与浓度的相关性。结果:与对照组比较,各实验组OD值均有明显下降。嗜酸乳杆菌活菌液实验组和20%悬液实验组不同稀释度OD值均低于阴性界值(<Cutoff)。10%悬液组稀释度>1∶64和5%悬液组稀释度>1∶256的OD值<Cutoff。混悬液组抑制效果与浓度相关。结论:嗜酸乳杆菌在体外实验中对HBeAg有明显的抑制作用,其抑制效果与浓度相关。
Objective： To investigate the inhibition effect of Lactobacillus acidophilus on HBeAg in vitro. Methods： First to get the positive serum of HBeAg for the samples,the test and control groups were designated. The liquid of living germs and the mixture dilutions of 5%, 10% and 20% Lactobacillus acidophilus were added into the test groups. At same time the blank liquids were added into control groups. After one hour at 37 ℃,the optical density （OD） values of each group were checked with ELISA. Finally the curves of the OD were drawn and the results were analyzed. Results： The values of OD in all the test groups were lower than that of the control group. The values of OD of the living germs and the 20% groups were under the level of the negative Cut-off value （〈Cut off）. The value of OD of 10% group 〉 1：64 and 5% group 〉 1：256 were less than Cut off. The inhibition effects on HBeAg were related to the concentrations of Lactobacillus acidophilus. Conclusion： Lactobacillus acidophilus could inhibit HBeAg effectively in vitro, which related to its concentration.
出处
《天津医药》
CAS
北大核心
2008年第1期44-45,共2页
Tianjin Medical Journal
关键词
乳杆菌
嗜酸
肝炎E抗原
乙型
酶联免疫吸附测定
抑制
化学性
体外研究
Lactobacillus acidophilus hepatitis B e antigens enzyme-linked immunosorbent assay depression, chemical in vitro
