摘要
目的通过观察大豆低聚糖对胃癌癌细胞株BGC-823细胞的细胞周期和细胞凋亡的影响,探索乳酸杆菌发酵滤液对胃癌细胞作用的可能机制。方法用光镜和流式细胞仪分析不同浓度大豆低聚糖对BGC-823细胞的凋亡诱导效果;用流式细胞仪分析不同浓度大豆低聚糖对BGC-823细胞细胞周期的影响。结果大豆低聚糖可以诱导BGC-823细胞的凋亡。形态学观察处理后的BGC-823细胞,可见细胞变形,细胞皱缩,体积变小,细胞间隙增大,细胞核固缩。流式细胞仪分析50 mg/ml和100 mg/ml大豆低聚糖作用48 h和72 h BGC-823细胞的凋亡比例,分别为6.76%和7.93%。50 mg/ml大豆低聚糖作用48 h,引起BGC-823细胞G1期阻滞,100 mg/ml大豆低聚糖作用48 h,引起BGC-823细胞出现S期阻滞。结论大豆低聚糖可诱导部分BGC-823细胞凋亡。大豆低聚糖对BGC-823细胞的生长抑制作用在低浓度时可能通过G1期阻滞实现,在高浓度时可能通过S期阻滞实现。
Objective To explore the effect of Soybean oligosaccharide on the cell cycle and apoptosis of gastric cancer cells BGC-823.Method Optical microscope and FCM were used to analyse the apoptosis of the different treated BGC-823 cells;FCM was used to analyse cell cycle of the different treated BGC-823.Result It had observed through microscope that the cell number had decreased and the cell shape was changed.The cells crimpled,their volume became smaller,and the space between the cells enlarged,with karyon pycnosis.This phenomenon was confirmed by FCM.Soybean oligosaccharide arrested BGC-823 in G1 phase at the concentration of 50 mg/ml,arrested BGC-823 in S phase at the concentration of 100 mg/ml.Conclusion Soybean oligosaccharide can induce apoptosis of some BGC-823 cells.It may inhibit the growth of BGC-823 cells through being arrested in G1 phase at low concentration while in S phase at high concentration.
出处
《中国微生态学杂志》
CAS
CSCD
2010年第5期404-405,410,共3页
Chinese Journal of Microecology
基金
国家自然科学基金(30670045)
国家"973"科学计划项目(2007CB513006)
国家"863"科学计划项目(2007A10Z356)
关键词
大豆低聚糖
胃癌细胞
细胞周期
细胞凋亡
Soybean oligosaccharide
Gastric cancer
Cell cycle
Cell Apoptosis
