摘要
通过对粳稻品种嘉花1号60Coγ射线诱变,从M2中筛选出一株低温敏感型白色条斑叶突变体(tws)。它在低温(20℃,24℃)条件下培养时,苗期第3和第4叶表现出白色条斑,而第5叶开始转为正常。低温条件下该突变体白斑叶片叶绿素含量明显下降。该突变体白色条斑叶性状具有温敏感性,且与叶龄相关。遗传分析表明,该突变性状受1对隐性核基因控制,定名为tws(thermo-sensitive white stripe-leaf)基因。以tws突变体与籼稻9311杂交的F2分离群体作为定位群体,利用SSR标记将该基因定位在第4染色体MM3907和MM3928之间,其物理距离约为86kb。
A thermo-sensitive white stripe-leaf mutant （tws） was selected from the M_2 progeny of a japonica rice variety, Jiahua 1, treated with 60Co γ-radiation. In comparison with the wild type parent （Jiahua 1）, the mutant displayed a phenotype of white stripe on the 3rd and 4th leaves, but began to turn a normal green phenotype on the 5th leaf when grown at low temperatures （20℃, 24℃）. And also the content of total chlorophyll showed an obvious decrease in the leaves with white stripe. These results suggest that the expression of the mutant trait was thermo-sensitive and correlated with the leaf age of seedlings. The genetic analysis indicated that the mutant trait was controlled by a single recessive nuclear gene, designated as tws （thermo-sensitive white stripe-leaf）. In addition, by using SSR markers and an F_2 segregating population derived from the cross between the tws mutant and 9311, the tws gene was mapped between MM3907 and MM3928 with a physical distance of 86 kb on chromosome 4.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2010年第3期223-227,共5页
Chinese Journal of Rice Science
基金
上海市科学技术委员会资助项目(08PJ14085
075405117
09DJ1400505
0939191200)
国家自然科学基金资助项目(30971552)
国家航天育种工程资助项目(2006HT100013)
关键词
水稻
温度敏感性
白色条斑叶
基因定位
rice（Oryza sativa）
thermo-sensitivity
white stripe-leaf
gene mapping
