摘要
目的探讨Toll样受体2(TLR2)对新生儿败血症诊断的临床意义,尤其对诊断早期症状不典型新生儿败血症的价值。方法选择22例败血症新生儿为研究对象,按照发病时间将患儿分为早发型组(n=12)及晚发型组(n=10);同时收集同期因非感染性疾病住院的14例新生儿为对照组。采集各组患儿外周血,应用密度梯度离心法分离出外周血单个核细胞,采用荧光定量PCR法检测TLR2 mRNA基因表达水平,并检测血清CRP水平。比较3组间各指标的差异,并用Pearson参数法分析TLR2 mRNA与CRP水平的相关性。结果 1.早发型组患儿CRP水平为(157.89±32.96)ng.L-1,晚发型组为(89.78±16.24)ng.L-1,二组比较有统计学差异(P=0.001);对照组CRP水平为(6.91±1.20)ng.L-1,明显低于早发型组及晚发型组(P=0.000,0.001)。2.早发型组、晚发型组患儿TLR2 mRNA基因表达水平分别为(57.38±10.72)×1012copies.L-1、(32.74±4.65)×1012copies.L-1,均明显高于对照组[(4.12±0.68)×1012copies.L-1]。3.CRP与TLR2呈明显正相关(C=0.972,P=0.000)。结论 TLR2在新生儿败血症的发病中起重要作用,其表达与CRP水平呈正相关,且与发病的早晚相关;早期检测TLR2可发现早期症状不典型的新生儿败血症。
Objective To explore the value of Toll-like receptor 2（TLR2） on the diagnosis of neonatal septicemia especially for the early diagnosis.Methods Twenty-two newborns with septicemia were divided into 2 groups according to the time of onset：early-onset group（n=12） and late-onset group（n=10）.Another 14 non-infectious newborns were collected as control group.The TLR2 mRNA in peripheral blood was detected by real-time polymerase chain reaction,and mononuclear cells were isolated by the density gradient centrifuga-tion;the C reaction protein（CRP） was detected,and the co-relationship of the indexes was analyzed by Pearson correlation analysis.Results 1.The level of CRP in early-onset group were（157.89±32.96） ng·L^-1and（89.78±16.24） ng·L^-1in late-onset group,and there was significant difference between both groups（P=0.001）;（6.91±1.20） ng·L^-1in control group,which was significantly lower than that in early-onset group and late-onset group（P=0.000,0.001）.2.The expression of TLR2 mRNA in early-onset group and late-onset group was（57.38±10.72）×1012 copies·L^-1nd（32.74±4.65） ×1012 copies·L^-1which was significantly higher than that in control group[（4.12±0.68） ×1012 copies·L^-1.3.CRP is positively correlated with TLR2 mRNA（C=0.972,P=0.000）.Conclusions TLR2 expression is proven to be related to both the level of CRP and the stage of onset and may play a key role in the pathogenesis of neonatal septicemia.Detection for TLR2 may assist the earlier diagnose of atypical neonatal septicemia.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2010年第10期741-742,745,共3页
Journal of Applied Clinical Pediatrics
