摘要
目的探讨外周血淋巴细胞表面标志物CD69在新生儿感染性疾病诊断中的价值。方法选取2007年3月-2008年12月在新生儿科(包括NICU)住院的患儿82例,分为重症感染组(42例)、轻症感染组(18例)和非感染组(22例)。应用流式细胞仪测定各组患儿外周血淋巴细胞CD69表达水平。应用SPSS 10.0软件进行统计学分析。结果非感染组、轻症感染组、重症组患儿外周血淋巴细胞CD69表达百分比分别为(1.13±0.69)%、(2.47±1.03)%、(2.16±1.24)%。轻症感染组患儿外周血淋巴细胞CD69表达水平显著高于非感染组(P=0.003),重症感染组患儿外周血淋巴细胞CD69表达水平显著高于非感染组(P=0.017),轻症感染组与重症感染组比较差异无统计学意义(P=0.546)。接受者操作特性曲线(ROC)计算得出CD69取临界值为2.19%,诊断新生儿感染的敏感度为42%,特异度为100%;CD69取临界值为1.29%,敏感度为83%,特异度为56%;CD69取临界值为0.99%,敏感度为92%,特异度为56%。结论新生儿感染时外周血淋巴细胞CD69表达水平明显升高,可作为早期诊断新生儿感染的实验室依据之一。
Objective To explore the diagnostic value of peripheral blood lymphocyte surface marker CD69 expression for neonatal infectious disease.Methods From Mar.2007 to Dec.2008,82 neonatal patients（including NICU patients） were divided into 3 groups：sepsic infection group（42 sepsic cases）,mild infection group（18 mild infectious cases） and non-infection group（22 non-infection cases）.The le-vels of lymphocyte CD69 were checked with flow cytometry.SPSS 10.0 software was used to analyze these data.Results The percentage of CD69 expression was（1.13±0.69）% in non-infection group,（2.47±1.03）% in mild infection group,（2.16±1.24）% in sepsic infection group.The percentage of CD69 expression in mild infection group was significantly higher than that in non-infection group（P=0.003）,and that in sepsic infection group was significantly higher than that in non-infection group（P=0.017）.There was no significant difference between sepsic infection group and mild infection group（P=0.546）.Receiver operating characteristic（ROC） curve was used to calculate the critical value.When the critical value of CD69 was 2.19%,the sensitivity of neonatal sepsis was 42% and the specificity was 100%;when the critical value of CD69 was 1.29%,the sensitivity was 83%,and specificity was 56%;when the critical value of CD69 was 0.99%,the sensitivity was 92% and the specificity was 56%.Conclusion Peripheral blood lymphocyte increase significantly in infectious diseases,and CD69 expre-ssion is a good laboratory mark to early diagnosis of neonatal infection.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2010年第22期1739-1740,共2页
Journal of Applied Clinical Pediatrics
基金
南京医科大学科技发展基金(06NMUM047)
