摘要
目的探讨肿瘤坏死因子配体相关分子1A(TL1A)在病毒性心肌炎(VM)小鼠中的表达及黄芪甲甙的干预作用。方法 55只雄性Balb/c小鼠随机分为4组:对照组、模型组、低剂量干预组及高剂量干预组。模型组、低剂量干预组、高剂量干预组小鼠经腹腔接种0.1 mL柯萨奇病毒B3建立VM模型,低剂量干预组、高剂量干预组小鼠于病毒接种后当天分别以10 g.L-1、90 g.L-1黄芪甲甙0.1 mL灌胃,对照组、模型组小鼠均以羧甲基纤维素钠溶液0.1 mL灌胃。实验第15天处死小鼠,取其心脏组织,石蜡包埋,切片,HE染色计算病理积分,免疫组织化学检测TL1A蛋白表达,反转录-聚合酶链反应检测TL1A mRNA的表达。结果对照组、模型组、低剂量干预组、高剂量干预组死亡率分别为0、46.7%、40.0%、13.3%,高剂量干预组死亡率明显低于模型组、低剂量干预组(χ2=9.46,8.95,Pa<0.05)。模型组心肌TL1AmRNA及TL1A蛋白表达水平均明显高于对照组(Pa<0.05),高剂量干预组病理积分及TL1AmRNA、TL1A蛋白表达量较模型组、低剂量干预组显著降低(Pa<0.05,0.01)。结论 TL1A参与VM发病过程。黄芪甲甙能明显下调TL1A表达,以减轻心肌损害,提高VM小鼠生存率。
Objective To explore the expression of tumor necrosis factor（TNF） ligand-related molecule-1A（TL1A） in mice with viral myocarditis（VM） and the role of astragaloside.Methods Fifty-five male Balb/c mice were randomly divided into 4 groups：control group,model group,low-dose intervention group and high-dose intervention group.Mice in model group,low-dose intervention group and high-dose intervention group were inoculated with 0.1 mL coxsackie B3 virus intraperitoneally.Then,mice in low-dose intervention group and high-dose intervention group were treated with 10 g·L^-1 and 90 g·L^-1 astragaloside solution,respectively.Mice in control group and model group were treated with 0.1 mL carboxymethycellulose solution.All mice were killed on the 15thday.Histological cross sections of heart were stained with hematoxylin-eosin and myocardial histopathologic scores were counted under optical microscope.The expressions of myocardial TL1A mRNA and protein were detected by reverse transcription polymerase chain reaction and immunohistochemistry.Results The mortality were 0,46.7%,40.0% and 13.3% in control group,model group,low-dose intervention group and high-dose intervention group,respectively.Compared with model group and low-dose intervention group,the mortality was significantly lower in high-dose intervention group（χ^2=9.46,8.95,Pa〈0.05）.The expression levels of TL1A mRNA and TL1A protein were markedly higher in model group than those in control group（Pa〈0.05）.However,those and myocardial histopathologic scores in high-dose intervention group were decreased markedly compared with those in model group and low-dose intervention group（Pa〈0.05,〈0.01）.Conclusions TL1A may participate in the pathogenesis of VM.Astragaloside can mardedly downregulate TL1A expression,attenuate myocardial damage and increase survival rate of mice with VM.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2010年第10期717-719,共3页
Journal of Applied Clinical Pediatrics
基金
国家自然科学基金(30271665)
