摘要
本实验采用20bp通用引物P3和P4的PCR方法对产A、D和E肠毒素的葡萄球菌进行了检测。结果表明,产SEE菌株能产生666bp的特异扩增片段,产SEA菌株产生666bp和约400bp两条扩增带,产SED菌株产生400bp片段;SEE菌株的扩增产物经EcoRV酶切能产生251和415bp两个片段。扩增敏感性实验表明,该方法可检出10~6个细菌。
Staphylococcal enterotoxigenicgenes entA,entD and entE were detected by universal primer PCR protocal.The Staphylococcal strains encoding enterotoxin A(SEA strains )could product two smplification fragments of 666 and 400bp.SED and SEE strains producted 400 and 666bp fragments respectively.After the PCR amplicons of SEE strain sere eleaved by restriction endonuclease EcoRV.electrophofretic analysis showed two 251 and 415bp DNA fragments.With the PCR method 10 enterohocigenic Staphylococcal cells couldbe detected.
基金
全军青年科研基金
关键词
通用引物
PCR
葡萄球菌
肠毒素基因
Universal primer PCR.Staphylococcal Enterotoxigenic gene
