摘要
目的:建立微波消解-氢化物发生原子荧光光谱测定人发中硒的方法。方法:发样经微波消解后,以抗坏血酸、硫脲为还原剂,用氢化物发生原子荧光光谱测定硒的浓度。结果:本法在硒浓度为0μg/L～10μg/L的范围具有良好的相关系数(r=0.9997),其最低检出浓度为0.088μg/L。应用此法对发样进行加标回收实验,加入量为2.5μg/L时,回收率范围为86.1%～101.9%,平均回收率92.8%;加入量为5.0μg/L时,回收率范围为85.4%～95.4%,平均回收率90.2%。结论:该法简便快速、灵敏度高、重现性好,适用于人发中微量硒的快速测定。
Objective：To establish a method for determination of selenium in hair by atomic fluorescence spectrometry with microwave digestion.Methods：Take the antiscorbutic acid,the thiourea as the reducing agent,determination of selenium in hair by atomic fluorescence spectrometry with microwave digestion.Results：The method had good linearity（r=0.9997） for the concentration of selenium from 0 μg/L to 10 μg/L,the detection limits were 0.088 μg/L.The recoveries were 86.1%～101.9% with an average of 92.8%（2.5 μg/L）,85.4%～95.4% with an average of 90.2%（5.0 μg/L）.Conclusion：This method is simple and rapid and had good accuracy and reproducibility.So it is suitable for the determination of selenium in hair.
出处
《中国卫生检验杂志》
CAS
2010年第5期1023-1024,共2页
Chinese Journal of Health Laboratory Technology
关键词
原子荧光光谱
发硒
Atomic fluorescence spectrometry
Hair selenium
