摘要
目的探讨人重组松弛素(rhRLX)对高糖环境大鼠心脏成纤维细胞胶原基因表达的影响。方法原代培养Sprague-Dawley大鼠心脏成纤维细胞。用RT-PCR方法分别观察在正常糖(NG,5.6mmol/L)和高糖(HG,25mmol/L)环境下二代成纤维细胞胶原基因和松弛素基因的表达;rhRLX(100μg/L)分别与NG,HG共同作用72h后成纤维细胞胶原基因的表达。结果HG促进Ⅰ型前胶原mRNA和Ⅲ型前胶原mRNA的表达,rhRLX可以抑制上述作用。HG组松弛素基因的表达比较明显。结论rhRLX对HG促进的Ⅰ型和Ⅲ型胶原基因的表达具有抑制作用。
Objective To determine the influence of recombinant human relaxin （rhRLX） on collagen gene expression in cardiac fibroblasts （CFs） under high glucose （HG） conditions. Methods CFs were isolated from Sprague-Dawley rats and cultured primarily. The mRNA levels of type Ⅰ,type Ⅲ collagen and RLX in CFs after 3 passages under normal glucose （NG,5.6 mmol/L） and high glucose （HG,25 mmol/L） conditions were assessed by RT-PCR. rhRLX （100 μg/L） was cocultured with CFs under NG and HG conditions for 72 h respectively,then the mRNA levels of type Ⅰand type Ⅲ collagen were detected. Results HG increased the expression of type Ⅰ and type Ⅲ collagen,which was inhibited by rhRLX. The RLX mRNA expression was stronger in HG group than in NG group. Conclusion rhRLX inhibits the HG-enhanced overexpression of typeⅠand type Ⅲ collagen.
出处
《中华老年多器官疾病杂志》
2010年第1期69-72,共4页
Chinese Journal of Multiple Organ Diseases in the Elderly
