摘要
目的：了解人参皂甙（ＧＳ）对巨核系祖细胞（ＣＦＵ－Ｍｅｇ）的刺激增殖作用，比较ＧＳ与生长因子ＴＰＯ、ＩＬ－３对ＧＡＴＡ转录调控蛋白的诱导作用。方法：正常人和ＩＴＰ患者骨髓ＣＦＵ－Ｍｅｇ培养和ＧＳ刺激试验、电泳带移动阻滞试验（ＥＭＳＡ）、抗体胶移动试验（Ｓｕｐｅｒｓｈｉｆｔ ａｓｓａｙ）和蛋白免疫印迹法（Ｗｅｓｔｅｒｎ Ｂｌｏｔ）。结果：ＧＳ刺激正常ＣＦＵ－Ｍｅｇ增殖，呈剂量依赖的方式，血小板减少性紫癜（ＩＴＰ）患者的ＣＦＵ－Ｍｅｇ也对ＧＳ敏感。ＧＳ、ＴＰＯ和ＩＬ－３均能诱导生长因子依赖ＭＯ７ｅ细胞核内ＧＡＴＡ蛋白与ＤＮＡ结合的活性及使ＧＡＴＡ－２蛋白含量增加。ＧＳ＋ＴＰＯ或ＧＳ＋ＩＬ－３可协同地诱导ＧＡＴＡ蛋白活性进一步增高。结论：ＧＳ能够通过类生长因子和增强生长因子作用而刺激ＣＦＵ－Ｍｅｇ增殖，ＧＡＴＡ族转录因子，主要是ＧＡＴＡ－２参与造血细胞对ＧＳ反应的信号传递途径。
Objective: To investigate the action of ginsenosides (GS) on megakaryocytic progenitors (CFU-Meg) and possible mechanisms. Methods:Using bone marrow culture of CFU-Meg,electrophoretic mobility shift assay (EMSA ), Supershift assay and Western blotting analysis. Results: GS promoted proliferation of CFU-Meg in a dose-dependent manner. CFU-Meg from patients with idiopathic thrombocytopenia purpura was response to GS.GATA binding activity and GATA-2 protein induced by GS was elevated in growth factor-dependent MO7e cells. GATA binding activity induced by the combination of GS with thrombopoietin (TPO),or GS with interleukin-3 (IL-3 ) were higher than those by GS, TPO,or IL-3 alone. Conclusions: GS may stimulate the proliferation of CFU-Meg by enhancing the effect of TPO or IL-3,and by its growth factor-like function,and GATA binding factors,mainly GATA-2,may mediate the response of megakaryocytes to GS.
出处
《临床血液学杂志》
CAS
1999年第2期50-54,共5页
Journal of Clinical Hematology
基金
中国和澳大利亚两国政府合作项目
澳大利亚政府科研基金
