摘要
将质粒ｐＢＲ３２２，ｃ－Ｍｙｃ，ｐＣ５３－ＳＮ３，ｐＴＢ２９转入大肠杆菌ＨＢ１０１中使其各自分别在含亚致死浓度Ｐｂ＾２＋，Ｃｄ＾２＋，Ｚｎ＾２＋，Ｃｕ＾２＋，Ｍｎ＾２＋，Ｈｇ＾２＋的液体氨苄ＬＢ培养基中，振荡培养２４ｈ，再将这些质粒抽提出来，通过限制性内切酶分析，研究了这些重金属离子对大肠杆菌体内质粒ＤＮＡ分子一级结构的影响。
Transformating the plasmids of PBR322. pC53-SN3, c-Myc and PTB29 into Escherichia coli HB101 andcultured with vibration in LB which contained separately sublethal concentration of Pb2+ ,Cd2+, Zn2+ ,Cu2+ ,Mn2+ orHg2+, constantly for 24 h,these plasmids were isolated.The effect of the heavy metals on plasmid DNA were studiedthrough the analysis of restriction fragment length polymorphism(RFLP). The result showed that only Hg2+ could lead tothe change of plasmid DNA sequence. Moreover, these heavy metals did not cause the random break of the double-strandplasmid DNA after the treatment in vivo of E. coli HB 101 for 24h.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
1999年第2期169-171,共3页
China Environmental Science
基金
云南省应用基础研究基金!95C144M
