摘要
目的：观察活血药川芎嗪、水蛭素、丹参酮ⅡＡ和凝血酶对高转移人巨细胞肺癌ＰＧＣＬ３（简称ＰＧＣＬ３）和低转移人肺腺癌ＰＡａ（简称ＰＡａ）细胞的粘附和侵袭的影响。方法：用不同浓度的川芎嗪、丹参酮ⅡＡ、水蛭素和凝血酶分别处理ＰＧＣＬ３和ＰＡａ细胞，观察ＰＧＣＬ３和ＰＡａ细胞单独或与血小板作用后对纤维粘连蛋白的粘附和Ｂｏｙｄｅｎ小室的侵袭改变。结果：川芎嗪、水蛭素和凝血酶在不同程度上增加ＰＧＣＬ３细胞的粘附，而丹参酮ⅡＡ抑制这种粘附。川芎嗪、丹参酮ⅡＡ和水蛭素抑制ＰＧＣＬ３细胞对Ｂｏｙｄｅｎ小室的侵袭，凝血酶明显促进这个过程。川芎嗪、丹参酮ⅡＡ、水蛭素抑制血小板与ＰＧＣＬ３和ＰＡａ细胞侵袭的协同作用，凝血酶对这个过程有促进作用。结论：活血药对肿瘤细胞的侵袭和转移可能既有抑制作用又有促进作用。
Objective: To investigate the influence of some blood activating drugs like Tetramethylpyrazine, Tanshinone ⅡA, Hirudin and thrombin on adhesive and invasive behavior of PGCL3 and PAa cell lines. Methods: Using the above mentioned blood activating drugs in various concentration to treat PGCL3 and PAa cells, and the changes in adhesion to fibronectin and invasion in Boyden Chamber of these cells, alone or after interacted with human platelets, were observed. Results: Tetramethylpyrazine, Hirudin and thrombin could increase the adhesion of cells to fibronectin and Tanshinone ⅡA decrease it. Tetramethylpyrazine, Tanshinone ⅡA and Hirudin inhibited the invasion of PGCL3 cells in Boyden Chamber, and thrombin augmented the process. Conclusion: The blood activating drugs may either inhibit or promote the invasion and metastasis of PGCL3 and PAa cells in the light of various conditions.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
1999年第2期103-105,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金
关键词
川芎嗪
水蛭素
凝血酶
肺癌细胞
粘附
侵袭
Tetramethylpyrazine, Hirudin, Tanshinone ⅡA, thrombin, lung cancer cell, adhesion, invasion
