摘要
目的:探讨土贝母苷甲(下称苷甲)对人高转移巨细胞肺癌PGCL3细胞粘附、侵袭和迁移能力的影响。方法:PGCL3细胞经不同剂量苷甲处理24、48、72h,MTT法检测其对PGCL3细胞生长的影响;分别用重组基底膜侵袭模型、粘附基质分析、Transwell小室趋化运动模型研究苷甲对PGCL3细胞的粘附、侵袭和运动能力的影响;用酶谱法检测苷甲对PGCL3细胞分泌Ⅳ型胶原酶(MMP-2)能力及活性的影响。结果:苷甲明显抑制PGCL3细胞的生长,且呈剂量依赖关系,作用24、48、72h的IC50分别为15.70、14.20和13.32μmo.lL-1。苷甲1.25、2.50、5.00μmol·L-1处理PGCL3细胞24h,对PGCL3细胞侵袭能力的抑制率分别为28.7%、41.0%、57.5%;对PGCL3细胞迁移能力的抑制率分别为21.6%、35.2%、53.7%。苷甲降低PGCL3细胞MMP-2的分泌量及其活性,降低PGCL3细胞对层粘连蛋白、纤维粘连蛋白的粘附率,且呈一定的剂量依赖关系。结论:苷甲抑制人高转移巨细胞肺癌PGCL3细胞的粘附、侵袭和迁移能力,其对PGCL3细胞的侵袭和粘附的影响可能与其降低PGCL3细胞MMP-2的分泌量及其活性,降低PGCL3细胞对层粘连蛋白、纤维粘连蛋白的粘附率有关。
AIM： To investigate the effects of tubeimoside 1 on adhesion, invasion and migration of PGCL3 ceils. METHODS： Cell growth inhibition mediated by tubeimoside 1 was measured by MTT assay. The effects of tubeimoside 1 on adhesion, invasion and migration of PGCL3 cells were determined using analysis of basement membrane components fibronectin and laminin, reconstituted basement membrane invasion assay, and Transwcll model, respectively. RESULTS： Tubeimoside 1 displayed growth inhibitory activity against PGCI3 cells with ICs0 values of 15.70, 14.20 and 13.32 μmol.L^-1 for 24, 48, 72 h of tubeimoside 1 treatment. 1.25, 2.50, 5.00μmol. L^-1 of tubeimoside 1 inhibited PGCL3 cells to invade the reconstituted basement membrane by 28.7%, 41.0% and 57.5%, and decreased the percentages of Iocomoting and migrating PGCL3 cells by 21.6%, 35.2% and 53.7%, respectively, in addition, tubeimoside 1 inhibited PGCL3 cells to adhere to the basement membrane components fibronectin and laminin, and the activity of MMP-2 and its secretion by PGCL3 cells in a dose-dependant manner. CONCLUSION： Tubeimoside 1 can significantly inhibit the adhesion, invasion and migration of PGC12 cells. The suppression of adhesion of PGCL3 cells to fibronectin and laminin and secretion and activity of MMP-2 in PGCL3 cells mediated by tubeimoside I are probably respon- sible for the inhibition of adhesion and invasion of PGCL3 cells.
出处
《中国天然药物》
SCIE
CAS
CSCD
2008年第2期135-140,共6页
基金
广东省自然科学基金(No.011809)~~
