摘要
目的探讨慢性铝染毒对大鼠脊髓的组织细胞学和β-淀粉样前体蛋白695(APP695)mRNA表达的影响及二者的关系。方法6月龄雌性SD大鼠20只,随机等分为正常组和染毒组。腹腔注射浓度为9mg/ml的AlCl3溶液染毒大鼠,每次1ml,每连续注射3天,间隔1天,共计72天。肉眼观察大鼠运动行为的变化。HE染色检测腰部脊髓灰质的组织细胞学变化。RT-PCR检测部脊髓APP695mRNA表达水平。结果与正常组比较,腹腔注射AlCl3溶液后,部分大鼠出现短时间的后肢运动障碍。慢性铝染毒大鼠腰部脊髓灰质内,嗜运动神经元现象明显,卫星现象明显,胶质细胞显著增多,横向神经纤维明显增粗增多,血管周围胶质细胞显著增多,灰质内空泡显著增多。慢性铝染毒大鼠脊髓APP695mRNA表达显著增强。结论三氯化铝溶液可造成短暂性大鼠后肢运动障碍。慢性铝染毒可导致脊髓神经元损伤和死亡,导致胶质细胞增多和灰质空泡变性等。APP695mRNA表达增高参与了上述脊髓神经元损伤过程。
Objective To study the effects of chronic triochloride aluminum（AlCl3） exposure on both histocytological changes and amyloid precursor protein 695（APP695） mRNA expression level of the rat spinal cord,in order to evaluate the relationship of the histocytological changes and the APP695 mRNA level in rat spinal cord exposed chronicly AlCl3.Methods Twenty six-month-old SD female rats were randomly divided into 2 groups,group normal and group exposed.The rats were exposed with intraperitoneal injection of 1ml AlCl3 solution for continuous 3 days of the interval of one day during the period of total 72 days.Behaviors of the rats were observed by naked eyes.Histocytological examinations of the spinal cords of the rats were performed with HE dyeing.The expression levels of APP695 mRNA of spinal cords were tested with RT-PCR.Results In comparition with group normal,intraperitoneal injection of AlCl3 solution caused movement obstacle of rat hind legs for short time.In comparition with normal group in the spinal cord cinerea of exposure group,invasion of glial cells into motor neuron appeared,satellite phenomenon appeared,glial cells prominently increased in number,traverse neurofibers prominently increased both in number and marking,glial cells around the blood vessels prominently increased in number,vacuoles prominently increased in number,expressions of APP695 mRNA prominently increased.Conclusion Intraperitoneal injection of AlCl3 solution could cause movement obstacle of rat hind legs for short time.Chronic aluminum exposure could cause injury or death of neurons,induced increases of glial cells,and vacuolar degeneration in rat spinal cords.Super expression of APP695 mRNA could participate the neuronal injury course.
出处
《卫生研究》
CAS
CSCD
北大核心
2010年第3期361-363,共3页
Journal of Hygiene Research
