摘要
本研究根据GenBank中已经发表的猪圆环病毒2型基因序列,设计了2对PCV2特异性引物,从疑似断奶仔猪多系统衰竭综合征(postweaning multisystemic wasting syndrome,PMWS)感染病例中检测出3株云南省PCV2流行株,通过ve-ro细胞分离病毒,并用透射电子显微镜观察到了约17nm的病毒样粒子的存在。经同源性比较分析,3个分离株之间核苷酸同源性为99.5%～99.8%,与甘肃省PCV2分离株核苷酸同源性最低(90.7%),浙江省分离株核苷酸同源性最高(99.7%),与南美洲分离株核苷酸同源性最低(92.0%),瑞典分离株核苷酸同源性最高(99.4%),这可能与云南省猪种引进有关。
According to the complete genome sequences of porcine circovirus type 2 （PCV2） published in GenBank,two pairs of primers were designed. The ORF2 genomes of three PCV2 isolates were amplified from the samples that were similar to those pigs with PMWS,and approximately 17 nm in diameter under electron microscopy. The results of homology showed that nucleotide homology between the three PCV2 isolates was 99.5%-99.8%,and were closely related to the Zhejiang isolate and the Gansu isolate,sharing 99.7% and 90.7%,and displayed 92.0% and 99.4% nucleotide homology with the Sweden isolate and the South America isolate,respectively.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第5期103-107,共5页
China Animal Husbandry & Veterinary Medicine
基金
云南省高端科技人才引培工程(2009CI125)项目资助
关键词
猪圆环病毒2型
分离鉴定
序列分析
porcine circovirus type 2
isolation and identification
sequence analysis
