摘要
为掌握江西地区猪圆环病毒2型(PCV2)的分子流行病学及其遗传变异情况,本研究运用实验室已建立的PCR方法对2013～2017年采集自江西省南昌市、宜春市、新余市、赣州市、吉安市、九江市、上饶市、抚州市、景德镇市、鹰潭市等10个地区的1 082份疑似PCV2感染猪病料组织进行病原学检测,并通过PCR扩增、克隆和基因测序对PCV2的全基因组序列进行分析。研究表明,1 082份病料中有610份为PCV2阳性,总阳性率为56.4%,其中2013～2017年阳性率分别为52.7%、48.8%、58.5%、71.0%和67.4%。共获得89株PCV2全基因组序列,其中有83株为PCV2b基因型,其中53株归类于PCV2b-1C基因亚群,30株归为PCV2b-1A/1B基因亚群;6株为PCV2a型。测序毒株与参考毒株ORF2基因核苷酸、氨基酸序列同源性分别为88.9%～100.0%和86.0%～100.0%;氨基酸序列分析表明,Cap蛋白存在20个氨基酸突变位点。本研究表明,江西地区猪群中PCV2的主导基因型为PCV2b,其中又以PCV2b-1C基因亚群占据主导地位,同时也存在少量PCV2a基因型。
To investigate the molecular epidemiology and genetic diversity of porcine circovirus type 2(PCV2)in Jiangxi province,1 082 tissue samples were collected during 2013 to 2017 from suspected pigs in 10 regions(including Nanchang,Yichun,Xinyu,Ganjiang,Ji’an,Jiujiang,Shangrao,Fuzhou,Jingdezhen and Yingtan cities)of Jiangxi province were detected for PCV2.And PCV2 isolates complete genome sequences were amplified,cloned and sequenced.The results showed that 610 of 1 082 samples were detected to be positive of PCV2 and the total positive rate was 56.4%.The positive rates from 2013 to 2017 were 52.7%,48.8%,58.5%,71.0%and 67.4%,respectively.The 83 of 89 PCV2 isolates belonged to PCV2b,and 53 and 30 of 83 isolates were grouped into PCV2b-1C and PCV2b-1A/1B,respectively,and 6 isolates belonged to PCV2a.The homology of nucleotides and the deduced amino acid sequence of ORF2 between sequenced strains and reference strains were 88.9%to 100.0%and 86.0%to 100.0%,respectively.Analysis of Cap acid amino sequences showed that there were 20 amino acid mutation sites.This research indicated that the dominant genotype of PCV2 in pigs in Jiangxi was PCV2b,and PCV2b-1C was a dominant subgenotype.At the same time,a small amount of PCV2a subgenotype also existed.
作者
蒋新华
周荷田
罗锋
杨丹凤
张志庆
陈松昌
邓舜洲
JIANG Xinhua;ZHOU Hetian;LUO Feng;YANG Danfeng;ZHANG Zhiqing;CHEN Songchang;DENG Shunzhou(Jiangxi Animal Disease Control Center,Nanchang 330096,China;Jiangxi Agricultural University,Nanchang 330045,China;Jiangxi Jinyibo Biotechnology Company,Nanchang 330013,China)
出处
《中国畜牧兽医》
CAS
北大核心
2019年第4期1143-1150,共8页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目(31460666)
江西省科技支撑计划项目(20132BBF60044
20141BBF60039)
江西省现代农业产业技术体系建设专项资金(JXARS-03)
关键词
江西省
猪圆环病毒2型(PCV2)
全基因组
分子流行病学调查
Jiangxi province
porcine circovirus type 2(PCV2)
complete genome
molecular epidemiological investigation
