摘要
目的探讨射线诱发肾癌细胞凋亡的作用和射线对肾癌细胞Ｐ５３，Ｂｃｌ－２基因表达的影响。方法利用末端脱氧核苷酰转移酶标记的流式细胞技术定性和定量分析射线诱导肾癌细胞凋亡。利用Ｐ５３和Ｂｃｌ－２单抗进行ＳＰＴＭ法免疫组织化学染色。结果０Ｇｙ、５Ｇｙ、１０Ｇｙ、１５Ｇｙ和２０Ｇｙ照射后细胞凋亡量分别为１．０２％、０．０３％、７．０３％、４．２２％和３６．０９％。０Ｇｙ照射组Ｐ５３表达标记指数为０．８０％，２０Ｇｙ照射后Ｐ５３表达明显增加达２３．８０％。０Ｇｙ照射组Ｂｃｌ－２基因表达为２４．４０％，２０Ｇｙ照射后Ｂｃｌ－２基因表达明显减少为１．８０％。结论低剂量Ｘ射线照射诱发肾癌细胞凋亡量很低，２０Ｇｙ时明显增加。射线诱发肾癌细胞凋亡伴随Ｐ５３基因表达增加和Ｂｃｌ－２基因表达减少，提示射线诱发细胞凋亡是多基因相互影响，相互作用的结果。
Objective\ To investigate the induction of apoptosis by X ray ionizing irradiation in Renal carcimona cell lines and the changes of X ray ionizing irradiation on the expression of P53 and Bcl 2 genes in Renal carcinoma cell lines. Method Immunohistological staining assay (SP TM assay) has been used for detect the changes of P53 and Bcl 2 gene expression after X ray ionizing irradiation. Results The percentages of apoptotic cells were 0.03%、7.03%、4.22% and 36.09% after ionizing irradiation of 5Gy、10Gy、15Gy and 20Gy respectively. Expression of P53 increased and Bcl 2 decreased significantly in 20Gy group. Conclution The percentages of apoptotic cells were very low with low dose radiation and increased with 20Gy radiation. The expression of P53 gene increased and Bcl 2 gene decreased greatly with 20Gy radiation than that of control group. It was indicated that the apoptosis induced by ionizing irradiation was regulated by some genes.
出处
《中国肿瘤临床与康复》
1999年第1期93-95,共3页
Chinese Journal of Clinical Oncology and Rehabilitation
