摘要
目的研究EPO对心肌缺血再灌注损伤的作用及其机制。方法实验大鼠随机分为假手术组、模型组、EPO组,冠脉结扎和再松开复制动物模型,计算心律失常评分和心肌梗死面积,免疫组化法检测心肌组织EPOR、NF-κB、TNF-α的表达。结果与假手术组、模型组比较,EPO组经EPO预处理后心律失常评分和心肌梗死面积减少,EPOR的表达增强(P<0.01)NF-κB、TNF-α的表达显著减少(P<0.01)。结论 EPO对心肌缺血再灌注损伤有明显保护作用,其作用机制可能与上调EPOR表达及下调NF-κB的表达有关。
Objective To study the protective effect of EPOR in ischemia-reperfusion injured myocardium and the mechanism. Methods Rats were randomly divided into three groups： sham operation group, model group, EPO group. Experimental animal model was conducted by ligating and loosening left anterior descending coronary. Arrhythmia score, infarct size, and the expression of EPOR, NF-κB, TNF-αwere compared in the three groups. The expression of EPOR, NF-κB, TNF-α was determined by immunohistochemical staining. Results In EPO group, the arrhythmia score, infarct size, the expression of NF-κB and TNF-α were significantly decreased than that in sham operation group and model group（P〈0.01）, while and the expression of EPOR was significantly increased.（P〈0.01）.Conclusion EPO could protect myocardium from ischemia-reperfusion injury by up-regulating the expression of EPOR and down-regulating the expression of NF-κB.
出处
《中国实用医药》
2010年第15期90-91,共2页
China Practical Medicine
