摘要
目的扩增大鼠细胞因子信号转导抑制因子-1（SOCS-1）基因，利用生物信息学方法预测其结构和功能特征，为进一步的实验研究提供理论指导。方法扩增并测序大鼠的s0CS-1编码区序列，利用生物信息学网站的在线分析工具和VectorNTIsuite软件包，识别大鼠s0CS-1基因并预测其编码蛋白质的各种结构特征，根据该基因构建其分子进化树。结果聚合酶链反应（PCR）扩增获得2个序列不同的SOCS-1基因，BLASTx分析该基因为全长基因，该基因全长639bp，编码212个氨基酸（aa），具有1个sOCS盒（aa172-aa208），1个Scr同源区2（sH2）结构域（aa80-aa155）和1个核定位序列（aa160～aa174），一级结构含有2个线性B细胞抗原表位，全部位于蛋白的表面，并在空间结构上相距较远。结论SOCS-1基因具有基因多态性，其保守的SH2区域及SOCS盒与其对信号转导通路抑制作用有关，而其核定位序列可能影响其他核转导因子，B细胞线性表位则在免疫诊断方面有较好的应用前景。
Objective To identify the suppressor of cytokine signaling-1 （SOCS-1） of rat from the amplified gene with the help of bioinformatics to predict the deduced protein＇s structure and function in order to lay the foundation for further theoretical study. Methods The full-length rat SOCS-1 gene was amplified and identified from the GeneBank Nucleotide database, and the corresponding structure and function of its deduced protein was predicted by the bioinformatics analyzing tools online and the complicated bioinformatics software package Vector NTI suite 8.0, meanwhile the molecular cladogram was reconstructed. Results Two sequences were obtained by polymerase chain reaction （PCR） amplification of different SOCS-1 gene. The gene was comprised of 639 base pairs in the length, deduced 212 amino acids （aa）, contained a SOCS box （aa172-aa208）, a SH2 domain （aaS0-aa155） and a nuclear localization sequence （aa160-aa174）. The primary structure contained two linear B cell epitopes, all of them were on the surface of the protein and far away from the spatial structure. Conclusion SOCS-1 gene has a polymorphism, its conservative SH2 region and SOCS box are related to its inhibitory effeet on the signal transduction pathway. The nucleic localization sequence may affect other nuclear transduction factors. The B cell linear epitopes may be a candidate of immunodiagnosis with promising prospects.
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2010年第5期259-262,共4页
Chinese Critical Care Medicine
基金
广东省产业技术研发计划项目（20098080701004）
第三军医大学创伤、烧伤与复合伤国家重点实验室开放基金资助项目（200711）
