摘要
目的观察细胞因子信号转导抑制因子-1（SOCS1）在脓毒症小鼠肝脏和脾脏中的变化情况，探讨SOCS在体内的可能作用机制。方法采用盲肠结扎穿孔术（CLP）制备脓毒症小鼠模型。提取肝脏和脾脏组织的总RNA及蛋白，采用逆转录-聚合酶链反应（RT—PCR）技术测定组织中SOCS1 mRNA的相对含量；用免疫印迹法测定组织中SOCS1相对蛋白含量；用SPSS统计软件分析上述指标之间的变化关系。结果脓毒症小鼠术后6h肝组织SOCS1的基因和蛋白表达均迅速升高，基因表达至24h达到高峰（P〈0．05），蛋白表达一直保持高位；在脾脏中仅检测到SOCS1基因表达，随时间延长逐渐上升，并一直保持高位；肝脏中SOCS1的基因和蛋白表达量间呈明显正相关（y=0．110±5．765×10＆-3x，r=0．837，F=93．309，P〈0．01）。结论CLP导致的脓毒症可诱导SOCS1在肝脏和脾脏中进一步表达，提示SOCS1在脓毒症出现后的免疫变化中有重要作用，可利用它们对脓毒症进行干预，以改善脓毒症的预后。
Objective To investigate the change in supressors of cytokine signaling - 1 （SOCS1） gene in the liver and the spleen of septic mouse, and to find out its probable mechanisms of action in sepsis. Methods Cecal ligation and puncture （CLP） was adopted to reproduce sepsis model. The liver and the spleen tissues were harvested and RNA and protein were respectively extracted. The contents of the regulatory genes SOCS1 mRNA were determined by reverse transcription -polymerase chain reaction （RT - PCR） and regulatory content of protein was detected by Western blotting. The SPSS statistics software was adopted to calculate the correlation. Results The expressions of SOCS1 on gene and protein in the liver were markedly upregulated at 6 th hour. The gene expression peaked at the 24 th hour （P〈0.05）. The expression of protein was persistently high. However, the expression of SOCS1 was only detected in the spleen, and it obviously rose in strength with the passage of time, and it remained in a high level. By statistical analysis, positive correlations were found between the gene and protein expressions of SOCS1 （y=0. 110±5. 765× 10^-3x, r=0. 837, F= 93. 309, P〈0.01）. Conclusion CLP induced sepsis can induce the up - regulation of the expressions of SOCS1, indicating that SOCS1 play important role in the change in immune system in sepsis. They may be used to intervene sepsis so as to improve the outcome of sepsis.
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2007年第10期606-609,共4页
Chinese Critical Care Medicine
基金
广东省广州市科委重点攻关课题（2001-Z-130-02）
广东省医学科研基金资助项目（A2003176）
