摘要
为观察腹腔感染（ＩＡＩ）致多系统器官功能障碍（ＭＳＯＤ）早期血浆内毒素和细胞因子的变化，在制作ＩＡＩ致ＭＳＯＤ动物模型的基础上，对感染组（行盲肠结扎加穿孔手术）及对照组（行盲肠探查术）兔在实验０、１、２、３、４、５、６及２４小时不同时相的血浆内毒素和细胞因子包括肿瘤坏死因子（ＴＮＦ）、白细胞介素１和６（ＩＬ１，ＩＬ６）进行了检测，并记录动物死亡情况和生存时间。结果显示：感染组血浆内毒素和ＴＮＦ水平在实验后１小时、ＩＬ６在实验后２小时开始显著升高，ＩＬ１无显著变化。感染组兔于１周内均死亡，平均生存时间为８４．１±３９．０小时，对照组兔１周时均存活，两组比较有显著性差异（Ｐ＜０．０５）。本实验所观测到的ＩＡＩ时血浆内毒素及细胞因子的早期变化特点为临床防治研究提供了实验基础。
To observe the change in plasma endotoxin and cytokine during the early period of intra abdominal infection (IAI) complicated by multiple system organ dysfunction (MSOD) in animals. Twenty rabbits were randomly divided in to two groups. One group received the operation of cecal ligation plus puncture (CLP) inducing IAI complicated by MSOD, and another group received sham operation as a control. All animals were placed in metabolic cages and maintained with intravenous infusion for one week. Plasma levels of endotoxin and cytokine (TNF, IL 1, IL 6) were determined seperately at the beginning (0 hour) or 1, 2, 3, 4, 5, 6 and 24 hours after CLP. Blood bacteria cultures and pathological examination of several organs were made when the animal was dead or killed. Results: The levels of plasma endotoxin, TNF and IL 6 were found to be significantly increased at one or two hours after CLP, the incidence rate of bacteriemia was 80% and the pathological alterations in the abdomen and organs were remarkale, with an average survival time of 84.1±39.0 hours in CLP group. No change in plasma IL 1 level was found in the CLP group. Conclusion: The plasma levels of endotoxin and cytokine (TNF and IL 6) do increase in the early period of IAI complicated by MSOD, and the change in plasma IL 1 is not obvious.
出处
《中国普外基础与临床杂志》
CAS
1999年第2期73-75,共3页
Chinese Journal of Bases and Clinics In General Surgery
关键词
腹腔感染
内毒素
细胞因子
多器官衰竭
Intra abdominal infection
Multiple system organ dysfunction
Endotoxin
Cytokine
Rabbit
