摘要
肺炎链球菌是严重侵袭性感染和上呼吸道感染最重要的条件致病菌之一,分析鉴定体内诱导基因序列变得尤为重要。本研究利用酶切自连法成功从129个体内诱导表达的肺炎链球菌中获得13个融合重组自杀质粒,通过与肺炎链球菌株TIGR4基因组序列的同源性比对,共得到10个不同的体内诱导基因,其中8个是从血液中筛选出的,另2个为从肺组织中筛选出;通过分析得到18个开放阅读框,其中大部分为已知功能基因,参与细菌多种生命活动,有2个为未知功能基因,编码假想蛋白。可见,酶切自连法可有效用于筛选基因的分析鉴定。
Streptococcus pneumoniae is one of the most important opportunistic pathogenas which can lead to serious invasive infection and upper respiratory tract infection. Thus,it became important to analysis and identification of in vivo induced genes sequences. In this study,the recombinant suicide plasmids were harvested by enzyme self-ligation method, and sequenced to obtain the in vivo induced genes sequences,the functions of which were predicted by bioinformatics analysis. 13 recombinant suicide plasmids,from the 129 individ- uals within the inducible expression of Streptococcus pneumoniae,were harvested. A total of 10 different in vivo induced genes were ob- tained by blasting with streptococcus pneumuniae TIGR4 genome sequence,8 of which are screened out from the blood,while the other two from the lung tissues were screened. By analyzing the available 18 open reading frames,most of which are known functional genes involved in a variety of life activities of bacteria. There were two genes of unknown function,encoded hypothetical proteins. The study proved that the enzyme self-ligation method can be effectively used to analysis and identification of screened gene fragments.
出处
《生物技术通报》
CAS
CSCD
北大核心
2010年第5期126-129,134,共5页
Biotechnology Bulletin
基金
国家自然科学基金面上项目(30970110)
关键词
酶切自连法
自杀质粒
肺炎链球菌
体内诱导基因
Enzyme Self-ligation method Suicide plasmid Streptococcus pneumuniae in vivo induced genes
