摘要
目的:寻找心肺复苏后大鼠血清硫化氢(H2S)浓度的变化规律以探讨H2S在心肺复苏(CPR)后病理生理发展中的作用。方法:将30只雄性SD大鼠随机分为对照组6只和实验组24只,实验组采用经皮电刺激心外膜建立大鼠心脏骤停(CA)模型,分别于CA前、自主循环恢复(ROSC)后2、4、6、8、10和12h采血测定H2S浓度并记录体温、平均动脉压、心率和呼吸频率,分析血清H2S在CPR后的动态变化规律及其与上述生命体征的相关性。结果:①实验组24只大鼠全部诱发心脏骤停并复苏成功,观察终点时(ROSC后12h)有14只大鼠存活且生命体征稳定;对照组无死亡现象。②实验组与对照组大鼠心肺复苏后血清H2S浓度动态变化趋势明显不同(F=12.226,P=0.003);实验组大鼠在心肺复苏后血清H2S浓度迅速升高,于ROSC后6h时达到高峰,然后回落至与对照组相似的水平。③血清硫化氢浓度与体温(偏相关系数r=-0.556,P=0.000)和平均动脉压(偏相关系数r=-0.240,P=0.002)呈负相关,而与心率和呼吸频率的相关性无统计学意义。结论:血清H2S变化可能是CA大鼠ROSC后代偿性反应的一种表现并参与体温和血压等生理过程的调节,但其确切机制还有待于进一步深入研究。
AIM：To investigate variation of hydrogen sulfide（H2S ） in serum of rats after cardiopulmonary resuscitation（CPR） and explore its pathophysiological role in CPR. METHODS：The 30 male SD rats were randomly divided into control group（n=6） and experimental group（n=24）,then models of cardiac arrest （CA） were established in rats of experimental group by transcutaneous electrical epicardium stimulation. Blood samples were collected before CA,at 2,4,6,8,10 and 12 h after restoration of spontaneous circulation （ROSC） for testing the serum levels of H2S,at the same time rectal temperature（Tr）,mean arterial pressure（MAP）,heart rate（HR）and respiration rate （R） were record. Next to analyze the dynamic variation of H2S content in serum of the rats after CPR and its correlation of the above-mentioned signs of life. RESULTS：（1）In experimental group,all of the 24 rats were successfully induced CA and resuscitation; at end points （12 h after ROSC） there were 14 rats were alive and their vital signs were stable. There was no death in the control group. （2）The variation trend of H2S content in serum was different between experimental group and the control（F=12.226,P=0.003）. In experimental group,H2S content in serum increased dramatically after CPR and reached peak at 6h after ROSC,then return to the similar level of the control group. （3） H2S content in serum was negatively correlated with Tr （partial r=-0.556,P=0.000） and MAP（partial r=-0.240,P=0.002）. But it wasn＇t correlated with HR and R（P〉0.05）. CONCLUSION：Change of H2S content in serum of rats after CPR may be a manifestation of compensatory responses after ROSC and participate in physiological processes such as body temperature and blood pressure regulation. But as for its precisely mechanisms,it still needs to be studied further.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2010年第4期363-365,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
广州市科技攻关计划项目(2004Z3-E0381)
关键词
心脏骤停
心肺复苏
自主循环恢复
硫化氢
cardiac arrest
cardiopulmonary resuscitation
restoration of spontaneous circulation
hydrogen sulfide
