摘要
研究上海地区不同人群中丙型肝炎病毒（ＨＣＶ）基因型的分布和频率。方法应用新改进的第二代谱线探针分析（ＬｉｎｅＰｒｏｂｅＡｓｓａｙ，ＬＩＰＡ）试剂盒，对１０９份血清的ＨＣＶＲＮＡ逆转录一巢式ＰＣＲ阳性分离物进行了基因分型。结果８１份丙型肝炎患者ＨＣＶ分离物中７１份（８６．７％）为ｌｂ型，４份（４．９％）为２ａ／２ｃ型，２份（２．５％）为３ｂ型，１份（１．２％）为６ａ型，２份（２．５％伪混合型（２ａ／２ｃ＋２ｂ＋１和ｌｂ＋６ａ），另有１份（１．２％）为未定型。７份献血员分离物中，５份为ｌｂ型，２份为２ａ型。但在２１份静脉吸毒者分离物中，８份（３８．１％）为ｌａ型，５份（２３８％）为ｌｂ型，２份（９．５％）为２ａ／２ｃ型，２份（９．５％）为３ａ型，１份（４．８％）３ｂ型，３份（１４．３％）为混合型（１ａ＋２ａ／２ｃ，ｌｂ＋２２ａ／２ｃ＋６ａ，１ａ＋ｌｂ＋３ｂ各１份）。结论（１）上海地区存在着分属４个ＨＣＶ基因型（１、２、３和６）的７个亚型（１ａ、ｌｂ、２ａ／２ｃ、２ｂ、３ａ、３ｂ和６ａ），其中２ｂ、３ａ、３ｂ和６ａ为首次发现。（２）ＨＣＶｌｂ型最常见于丙型肝炎患者和献血员。１ａ和３ａ仅见于静脉吸毒者。（３）静脉吸?
Objective To investigate the distribution and frequency of various grnotypes in differentpopulations in Shanghai. Methods 109 HCV isolates by using RT-nested PCR including 81 from patientswith hepatitis C (PHCs), 7 from blood donors (BDs) and 21 from introvenous drug abusers (IVDAs) weregenotyped by using recently improved second generation line probe assay (INNO-LIPA Hey ninnogenetics N. V .Belgium). Results Among 81 HCV isolates from PHCs, there were 71 (87.6%) genotypelb, 4 (4.9.%)2a, 2(2.5%) 3b, 1 (l.2%)6a, 2 (2.5%) mixed genotypes (l for 2a or 2c+2b+l, 1 for lb+6a) and 1(l.2%) undeterminable resistively, and among 7 from BDs, there were 5 lb and 2 g6notype 2a. Butamong 21 from IVDAs, there were 8(38.l%) gnotype la, 5 (23.8%) lb, 2(9.5%)2a or 2c, 2 (9.5%) 3a, I (4.8%)3b and 3 (14.3%) mixed genotypes (l for la+2a or 2c, 1 for lb+2a or 2c+6a and I for la+lb+3b)respectively. Conclusion (l)7 HCV Subtypes (la, fo, 2a, 2b, 3a, 3b and 6a) belonging to 4 HCV genotypes(l, 2, 3, 6) were present in different populations in Shanghai. Of them, 2b, 3a, 3b and 6a were firstfound. (2)HCV genotype lb was most commonly found in PHCs and BDs. la and 3a were only found inIVDAs. (3)Tile fact that more HCV subtypes were found in IVDAs in this city.
出处
《中华肝脏病杂志》
CAS
CSCD
1999年第1期29-30,共2页
Chinese Journal of Hepatology
