摘要
目的探讨Rho激酶抑制剂和解除狭窄联合治疗重度颈动脉狭窄所致的认知功能障碍的疗效。方法取造模成功的60只SD大鼠随机选择其中48只分为药物组,法舒地尔8.35 mg/(kg·d),狭窄解除组,联合组(解除狭窄联合法舒地尔治疗)和对照组,每组12只。另选6只大鼠不结扎颈动脉为假手术组。各组以不同干预措施处理2周后,进行Morris水迷宫行为学检测大鼠的认知功能,并观察大鼠海马神经病理形态学,比较治疗前后颈动脉狭窄率。结果治疗后,与对照组比较,3个治疗组大鼠平均逃避潜伏期明显缩短(P<0.01),联合组较药物组和狭窄解除组改善更明显(P<0.05);狭窄解除组和联合组大鼠颈动脉狭窄率明显下降(P<0.01)。联合组狭窄率较药物组和狭窄解除组明显下降(P<0.01)。各组海马组织病理形态学变化有不同程度的改善。结论联合治疗可明显改善重度颈动脉狭窄导致的认知功能障碍,疗效优于单纯的药物治疗或解除狭窄治疗。
Objective To explore the efficacy of Rho kinase inhibitor and relieving stenosis in the treatment of cognitive dysfunction induced by severe carotid artery stenosis. Methods SD rats were used to establish severe carotid artery stenosis model. After 2 weeks, they were randomly di- vided into four groups：drug treatment group（receiving intraperitoneal injection of fasudil）, relie- ing stenosis group, combined treatment group（relieving stenosis plus fasudil treatment）, control group. Other 6 rats without carotid artery ligation served as sham-operated group. After different interventions for 2 weeks,Morris water maze test was used to observe changes in cognitive func- tion of the rats and the pathomorphology of rat hippocampus was examined to compare the rate of carotid artery stenosis before and after treatment. Results After treatment, compared with con- trol group,the mean escape latency in rats of other 3 groups significantly reduced （P 〈 0.01）, combined treatment group was significantly improved compared with simple drug treatment and stenosis relieving groups （P ~ 0.05）. Compared with control group,the rate of carotid stenosis in stenosis relieving and combined treatment groups was significantly decreased （P ~ 0.01）. The de- crease was more significant in combined therapy than in simple drug treatment and stenosis relie- ving groups （P 〈 0.01）. Varying degrees of improvement of pathological changes in hippocampus were observed in 3 treated groups. Conclusion Relieving carotid stenosis and inhibition of Rho- kinase can markedly improve cognitive impairment,and the efficacy of the combined treatment is superior to single treatment.
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2010年第5期465-468,共4页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
