摘要
目的:揭示莪术油对小鼠肝癌细胞增殖的抑制机理。方法:用莪术油进行2次抑制小鼠肝癌HepA实验,用病理细胞图像分析仅分析莪术油对小鼠肝癌细胞核DNA含量的影响。结果:莪术油对小鼠肝癌HepA的抑瘤率分别为51.85％和51.16％,与对照组比较有显著性差异(P<0.01)。莪术油能降低小鼠HepA肝癌细胞的DNA光密度值(249.28±70.53∶430.23±160.06,P<0.005)、核面积(623.48±227.65∶1073.29±100.99μm^2,P<0.005)及DNA指数(2.376±0.672∶4.481±0.706,P<0.001),同时能提高肝癌细胞中二倍体细胞的比例(29.00±9.345∶2.97±5.69％,P<0.001)及降低超五倍体细胞比例(30.04±15.10％∶70.89±14.94％,P<0.001)。结论:莪术油具抗肝癌作用,其机理可能与其抑制肿瘤细胞DNA合成有及增殖活性有关。
Aim:To reveal the inhibitory mechnism of CAO on the cellular proliferation of hepatic carcinomas carried by mice. Methods: Two inhibitory experiments of CAO on Hep A liver cancer were carried out in mice. With DNA image cytometry. we analyzed the effect of CAO on cellular nuclear DNA contents of hepatic carcinoma carried by mice. Results: The tumor inhibitory rates of CAO were 51. 85% and 51. 16% in two experiments, respectively. Compared with those of the saline-treated control groups, both differences were statisticaly significant (P<0. 01). In the group of mice treated with CAO. the cellular nuclear DNA OD ralue, areas and DNA index of hepatic were 249. 28±70. 53. 623. 48 ±227. 65 and 2. 376±0.672 respectively, which were significantly lower than those of the control group (430. 23±160.06,1073. 29±100. 99 and 4. 481±0. 706). CAO also could increase dipioidy cell rates(29. 00±9. 34 vs 2. 97±5. 69% (P<0. 01), and decrease pentaploidy cell exceeding rate (30. 04± 15. 10 vs 70. 89±14.94%±14. 94 % , P<0. 001). Conclusion: The mechanism of inhibitory effect of CAO ob Hep A liver cancer in mice mighe beassociated with inhibition of DNA synthesis and cell proliferation.
出处
《中西医结合肝病杂志》
CAS
1999年第1期18-20,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
国家"九五"科技攻关课题
广东省中医药管理局课题
关键词
莪术油
肝癌
DNA
图像分析
Curcuma Aramatica Oil Liver Cancer DNA Image Cytometry
