摘要
为研究东亚钳蝎毒灌肠治疗大肠癌前后肿瘤组织中DNA参数的变化,采用CMIAS真彩色医学图像分析系统对20例大肠癌患者用东亚钳蝎毒灌肠治疗后肿瘤细胞核DNA参数测定,结果发现:①DNA指数均值(DI):治疗前DI值1.92±0.19。治疗后DI值1.58+0.24,治疗后同治疗前比较DI值明显缩小(P<0.01)。②DNA倍体类型的分布:治疗后与治疗前比较,≥5倍体明显减少,二倍体明显增多(P<0.05)。③细胞核形态参数:治疗后与治疗前比较,细胞核面积的均值,核周长的均值和细胞浆面积均值明显减少,有显著性差异(P<0.05)。同期随机选10例直肠癌患者用氟脲嘧啶灌肠,治疗前后各参数值虽有变化,但无统计学意义(P>0.05)。说明东亚钳蝎毒对大肠癌有抑杀作用,且优于氟脲嘧啶。
By using CMTAS color graphic analysis, DNA parameters in the tumor nucleus were determined in 20 patients with rectum carcimona after the enema treatment by scorpion venom of buthus marten-sii Ranch. The statistic results showed that the mean value of DNA index (DT) decreased significantly (P< 0. 01), with the DT value being 1.92±0.19 before the treatment and 1.58±0.24 aftter the treatment, respectively. The comparison before and after the treatment showed that multiploidy (≥5) was obviously decreased and the ploidy obviously increased (P<0. 05). The nucleus morphological index:Before the treatment, the nucleus morphological indices including the mean nucleus area, cytoplasm area and the average nucleus circumference were markedly decreased as compared with those after the treatment with the difference being significant (P<0. 05). 10 cases of rectum carcinoma were randomly selected as controls treated by fluorouracilum enama,with the various paramenters without statistivalsignificance (P>0. 05). From above, it was concluded that the control of the scorpion venom of Buthus Martensii Kanch on rectum carcinoma was superior to that of fluorouracilum.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1997年第3期144-145,共2页
Chinese Journal of Experimental Surgery
关键词
大肠肿瘤
东亚钳蝎毒
中药灌肠
DNA指数
CMTAS analysis
scorpion venom of Buthus Martensii Kanch
fluorouracilum
DNA index
rectum carcinoma
